STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30]
Catalog# HA724771
STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30]
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WB
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FC(Intra)
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
STIM2 Recombinant Rabbit Monoclonal Antibody [PSH28-30]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human STIM2 aa 401-746.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, FC(Intra)
Target Molecular Weight
Predicted band size: 84 kDa
Positive Control
HEK-293 (Human embryonic kidney cell) cell lysate, BT-20 (Human breast cancer cell) cell lysate, MDA-MB-231 (Human breast cancer cell) cell lysate, MCF7 (Human breast cancer cell) cell lysate, HeLa (Human cervical adenocarcinoma cell) cell lysate, PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate, COS-1 (African green monkey kidney fibroblast) cell lysate, Mouse spleen tissue lysate, Mouse brain tissue lysate, Rat spleen tissue lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
PSH28-30
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC(Intra) | IF-Cell | |
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| human |
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| mouse |
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| rat |
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| green monkey |
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Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:2,000
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FC(Intra)
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1:200
Target
Function
Stromal interaction molecule 2 (STIM2) is a protein that in humans is encoded by the STIM2 gene. This gene is a member of the stromal interaction molecule (STIM) family which comprises only two members together with its homologue STIM1, and likely arose from a common ancestral gene. They encode type 1 transmembrane proteins that are located in the sarco/endoplasmic reticulum (SR / ER) into the cell. Alternative translation initiation from an AUG and a non-AUG (UUG) start site results in the production of two different STIM2 isoforms.
Background References
1. Chhikara A et al. Activity-dependent localization and dynamics of STIM1 and STIM2 at ER-PM contacts in hippocampal neurons. Cell Rep. 2025 Oct
2. Luo R et al. STIM2 variants regulate Orai1/TRPC1/TRPC4-mediated store-operated Ca(2+) entry and mitochondrial Ca(2+) homeostasis in cardiomyocytes. Cell Calcium. 2024 May
Subcellular Location
Endoplasmic reticulum membrane.
Synonyms
FLJ39527 antibody
KIAA1482 antibody
STIM 2 antibody
STIM2 antibody
STIM2_HUMAN antibody
Stromal interaction molecule 2 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cell) cell lysate
Lane 2: BT-20 (Human breast cancer cell) cell lysate
Lane 3: MDA-MB-231 (Human breast cancer cell) cell lysate
Lane 4: MCF7 (Human breast cancer cell) cell lysate
Lane 5: T-47D (Human breast ductal carcinoma cell) cell lysate
Lane 6: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 7: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lane 8: COS-1 (African green monkey kidney fibroblast) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Low expression: T-47D (PMID: 31464639)
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 84 kDa
Observed band size: 100 kDa -
Western blot analysis of STIM2 on different lysates with Rabbit anti-STIM2 antibody (HA724771) at 1/2,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat spleen tissue lysate
Lane 4: Rat brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724771, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 84 kDa
Observed band size: 100 kDa -
Application: Flow Cytometry (Intra)
Species: Human
Sample: HEK-293 (Human embryonic kidney cell)
Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
Permeabilization: 0.1% Tween-20, 15 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature.
Antibody dilution buffer: 1x PBS.
Primary antibody: HA724771 (1/200, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature.
Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"