TCF21 Rabbit Polyclonal Antibody
Catalog# HA500114
TCF21 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TCF21 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human TCF21 aa 52-90.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 20 kDa
Positive Control
293T cell lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse brain tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:500-1:2,000
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IHC-P
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1:100-1:500
Target
Function
TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene. Involved in epithelial-mesenchymal interactions in kidney and lung morphogenesis that include epithelial differentiation and branching morphogenesis. May play a role in the specification or differentiation of one or more subsets of epicardial cell types.
Background References
1. Ao X. et. al. TCF21: a critical transcription factor in health and cancer. J Mol Med (Berl). 2020 Aug
2. Nagao M. et. al. Coronary Disease-Associated Gene TCF21 Inhibits Smooth Muscle Cell Differentiation by Blocking the Myocardin-Serum Response Factor Pathway. Circ Res. 2020 Feb
Subcellular Location
Nucleus.
Synonyms
bHLHa23 antibody
Capsulin antibody
Class A basic helix-loop-helix protein 23 antibody
Epicardin antibody
POD 1 antibody
Pod-1 antibody
POD1 antibody
Podocyte expressed 1 antibody
Podocyte-expressed 1 antibody
TCF-21 antibody
ExpandImages
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Western blot analysis of TCF21 on different lysates with Rabbit anti-TCF21 antibody (HA500114) at 1/1,000 dilution.
Lane 1: 293T cell lysate (20 µg/Lane)
Lane 2: Mouse kidney tissue lysate (40 µg/Lane)
Lane 3: Rat kidney tissue lysate (40 µg/Lane)
Predicted band size: 20 kDa
Observed band size: 26 kDa
Exposure time: 14 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500114) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TCF21 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA500114, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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