TCF21 Rabbit Polyclonal Antibody
Catalog# HA500188
TCF21 Rabbit Polyclonal Antibody
-
WB
-
Human
-
unconjugated
Overview
Product Name
TCF21 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human TCF21 aa 125-175.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 20 kDa
Positive Control
Hela cell lysate, 293T cell lysate, K562 cell lysate, human lung tissue lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:500-1:1,000
Target
Function
TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene.
Background References
1. Nagao M. et. al. Coronary Disease-Associated Gene TCF21 Inhibits Smooth Muscle Cell Differentiation by Blocking the Myocardin-Serum Response Factor Pathway. Circ Res. 2020 Feb
2. Wirka RC. et. al. Atheroprotective roles of smooth muscle cell phenotypic modulation and the TCF21 disease gene as revealed by single-cell analysis. Nat Med. 2019 Aug
Subcellular Location
Nucleus.
UNIPROT
Synonyms
bHLHa23 antibody
Capsulin antibody
Class A basic helix-loop-helix protein 23 antibody
Epicardin antibody
POD 1 antibody
Pod-1 antibody
POD1 antibody
Podocyte expressed 1 antibody
Podocyte-expressed 1 antibody
TCF-21 antibody
ExpandImages
-
Western blot analysis of TCF21 on k562 cell lysate with Rabbit anti-TCF21 antibody (HA500188) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA500188, 1/1,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/20,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 20 kDa
Observed band size: 20 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
