TCPTP Recombinant Rabbit Monoclonal Antibody [JE57-07]
Catalog# HA720074
TCPTP Recombinant Rabbit Monoclonal Antibody [JE57-07]
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WB
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FC
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
TCPTP Recombinant Rabbit Monoclonal Antibody [JE57-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TCPTP aa 1-100/415.
Species Reactivity
Human, Mouse (Predicted: Rat)
Predicted species support after-sales service
Validated Applications
WB, FC
Molecular Weight
Predicted band size: 48 kDa
Positive Control
K562 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, HL-60 cell lysate, Jurkat cell lysate, Daudi.
Conjugation
unconjugated
Clone Number
JE57-07
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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FC
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1:500-1:1,000
Target
Function
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.
Background References
1. Spalinger MR. et. al. PTPN2 Regulates Interactions Between Macrophages and Intestinal Epithelial Cells to Promote Intestinal Barrier Function. Gastroenterology. 2020 Nov
2. LaFleur MW. et. al. PTPN2 regulates the generation of exhausted CD8(+) T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019 Oct
Subcellular Location
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus.
Synonyms
Protein tyrosine phosphatase non receptor type 2 antibody
PTN2 antibody
PTN2_HUMAN antibody
PTPN 2 antibody
Ptpn2 antibody
PTPT antibody
T cell protein tyrosine phosphatase antibody
T-cell protein-tyrosine phosphatase antibody
TC PTP antibody
TCELLPTP antibody
ExpandImages
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Western blot analysis of TCPTP on different lysates with Rabbit anti-TCPTP antibody (HA720074) at 1/5,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA720074, 1/5,000 in primary antibody dilution buffer 5% NFDM/TBST, overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 48.5 kDa
Observed band size: 45, 48 kDa -
Flow cytometric analysis of Daudi cells labeling TCPTP.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA720074, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14]
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
Products with the same target and pathway
TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated