TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14]
Catalog# HA724425
TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14]
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WB
-
IHC-P
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
HCT 116 (Human colon cancer cells) cell lysate, RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate, NIH/3T3 (Mouse fibroblasts) cell lysate, NR8383 (Rat alveolar macrophages) cell lysate, COS-1 (African green monkey kidney fibroblasts) cell lysate.
Conjugation
unconjugated
Clone Number
PSH24-14
Product Features
Form
Liquid
Concentration
0.5 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:10,000
-
IHC-P
-
1:500
Target
Function
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. Epidermal growth factor receptor and the adaptor protein Shc were reported to be substrates of this PTP, which suggested the roles in growth factor mediated cell signaling. Multiple alternatively spliced transcript variants encoding different isoforms have been found. Two highly related but distinctly processed pseudogenes that localize to chromosomes 1 and 13, respectively, have been reported.
Background References
1. Spalinger MR. et. al. PTPN2 Regulates Interactions Between Macrophages and Intestinal Epithelial Cells to Promote Intestinal Barrier Function. Gastroenterology. 2020 Nov
2. LaFleur MW. et. al. PTPN2 regulates the generation of exhausted CD8(+) T cell subpopulations and restrains tumor immunity. Nat Immunol. 2019 Oct
Subcellular Location
Cell membrane, Cytoplasm, Endoplasmic reticulum, Membrane, Nucleus.
Synonyms
Protein tyrosine phosphatase non receptor type 2 antibody
PTN2 antibody
PTN2_HUMAN antibody
PTPN 2 antibody
Ptpn2 antibody
PTPT antibody
T cell protein tyrosine phosphatase antibody
T-cell protein-tyrosine phosphatase antibody
TC PTP antibody
TCELLPTP antibody
ExpandImages
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Western blot analysis of TCPTP on different lysates with Rabbit anti-TCPTP antibody (HA724425) at 1/10,000 dilution.
Lane 1: HCT 116 (Human colon cancer cells) cell lysate
Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cells) cell lysate
Lane 3: NIH/3T3 (Mouse fibroblasts) cell lysate
Lane 4: NR8383 (Rat alveolar macrophages) cell lysate
Lane 5: COS-1 (African green monkey kidney fibroblasts) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 17 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724425, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 48 kDa
Observed band size: 45 kDa -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Mouse
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Heart
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature. -
Application: Immunohistochemistry (IHC-P)
Species: Rat
Tissue: Colon
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA724425, 1/500, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
TCPTP Recombinant Rabbit Monoclonal Antibody [PSH24-14] - BSA and Azide free
Application: WB,IHC-P
Reactivity: Human,Mouse,Rat,Green monkey
Conjugate: unconjugated
