Skip to content

TIM 3 Mouse Monoclonal Antibody [1E6]

Usd: 350

Specification

Bulk Order Quote | Customization Request

Catalog# EM1701-18

TIM 3 Mouse Monoclonal Antibody [1E6]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TIM 3 Mouse Monoclonal Antibody [1E6]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Recombinant protein with Human TIM 3 1-89 / 301.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 33 kDa

Positive Control

293T cell lysate, 293T, human tonsil tisssue, human lung cancer tissue, human spleen tissue, Daudi.

Conjugation

unconjugated

Clone Number

1E6

RRID

AB_3068746

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2000

  • IF-Cell

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

Target

Function

Cell surface receptor implicated in modulating innate and adaptive immune responses. Generally accepted to have an inhibiting function. Reports on stimulating functions suggest that the activity may be influenced by the cellular context and/or the respective ligand. Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. In CD8+ cells attenuates TCR-induced signaling, specifically by blocking NF-kappaB and NFAT promoter activities resulting in the loss of IL-2 secretion. The function may implicate its association with LCK proposed to impair phosphorylation of TCR subunits, and/or LGALS9-dependent recruitment of PTPRC to the immunological synapse.

Background References

1. Weber JK et al. Phosphatidylserine-Induced Conformational Modulation of Immune Cell Exhaustion-Associated Receptor TIM3. Sci Rep 7(1):13579 (2017).

2. Delpoux A et al. FOXO1 opposition of CD8+ T cell effector programming confers early memory properties and phenotypic diversity. Proc Natl Acad Sci U S A 114(42):E8865-E8874 (2017).

Sequence Similarity

Belongs to the immunoglobulin superfamily. TIM family.

Tissue Specificity

Expressed in T-helper type 1 (Th1) lymphocytes. Expressed on regulatory T (Treg) cells after TCR stimulation. Expressed in dendritic cells and natural killer (NK) cells. Expressed in epithelial tissues. Expression is increased on CD4+ and CD8+ T-cells in chronic hepatitis C virus (HCV) infection. In progressive HIV-1 infection, expression is up-regulated on HIV-1-specific CD8 T-cells.

Post-translational Modification

O-glycosylated with core 1 or possibly core 8 glycans.; Phosphorylated on tyrosine residues; modestly increased after TCR/CD28 stimulation. Can be phosphorylated in the cytoplasmatic domain by FYN (By similarity). Phosphorylation at Tyr-265 is increased by stimulation with ligand LGALS9.

Subcellular Location

Membrane. Cell junction.

UNIPROT

SwissProt: Q8TDQ0 Human

Synonyms

CD366 antibody

FLJ14428 antibody

HAVcr-2 antibody

Havcr2 antibody

HAVR2_HUMAN antibody

Hepatitis A virus cellular receptor 2 antibody

Kidney injury molecule 3 antibody

KIM 3 antibody

KIM3 antibody

T cell immunoglobulin and mucin domain containing 3 antibody

Expand

Images

  • Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/100 dilution.

    Western blot analysis of tim3 on 293T cell lysate using anti-Tim3 antibody at 1/100 dilution.

  • Immunocytochemistry analysis of 293T cells labeling TIM 3 with Mouse anti-TIM 3 antibody (<a href="/products/EM1701-18" style="font-weight: bold;text-decoration: underline;">EM1701-18</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-TIM 3 antibody (<a href="/products/EM1701-18" style="font-weight: bold;text-decoration: underline;">EM1701-18</a>) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of 293T cells labeling TIM 3 with Mouse anti-TIM 3 antibody (EM1701-18) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-TIM 3 antibody (EM1701-18) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Tim3 antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation