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Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]

Usd: 350

Specification

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Catalog# HA601028

Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Topoisomerase II alpha Mouse Monoclonal Antibody [A3F12]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Topoisomerase II alpha aa1480-1531.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 174 kDa

Positive Control

Jurkat cell lysate, A549 cell lysate, NIH/3T3 cell lysate, human breast carcinoma tissue.

Conjugation

unconjugated

Clone Number

A3F12

RRID

AB_3071752

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein G affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:200

Target

Function

DNA topoisomerase IIα is a human enzyme encoded by the TOP2A gene. Topoisomerase IIα relives topological DNA stress during transcription, condenses chromosomes, and separates chromatids. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several chemotherapy agents and a variety of mutations in this gene have been associated with the development of drug resistance.[citation needed] Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. TOP2A has been shown to interact with SMURF2, HDAC1, CDC5L, Small ubiquitin-related modifier 1 and P53.

Background References

1. Wyles J.P. et. al. Nuclear interactions of topoisomerase II alpha and beta with phospholipid scramblase 1. Nucleic Acids Res. 35:4076-4085(2007).

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: P11388 Human

SwissProt: Q01320 Mouse

SwissProt: P41516 Rat

Synonyms

alpha isozyme antibody

ATP hydrolyzing DNA topoisomerase II alfa antibody

DNA gyrase antibody

DNA topoisomerase (ATP hydrolyzing) antibody

DNA topoisomerase 2 alpha antibody

DNA topoisomerase 2-alpha antibody

DNA topoisomerase II 170 kD antibody

DNA topoisomerase II alpha isozyme antibody

DNA topoisomerase II antibody

DNA Topoisomerase2 antibody

Expand

Images

  • Western blot analysis of Topoisomerase II alpha on Jurkat cell lysates with Mouse anti-Topoisomerase II alpha antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 174 kDa<br />Observed band size: 174 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Topoisomerase II alpha on Jurkat cell lysates with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 174 kDa
    Observed band size: 174 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601028) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Topoisomerase II alpha on different cell lysates with Mouse anti-Topoisomerase II alpha antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/500 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: NIH/3T3 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 174 kDa<br />Observed band size: 174 kDa<br /><br />Exposure time: 2 minutes;<br /><br />6% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:20,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Topoisomerase II alpha on different cell lysates with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution.

    Lane 1: A549 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: NIH/3T3 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 174 kDa
    Observed band size: 174 kDa

    Exposure time: 2 minutes;

    6% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601028) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Topoisomerase II alpha antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA601028" style="font-weight: bold;text-decoration: underline;">HA601028</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Topoisomerase II alpha antibody (HA601028) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601028) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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metafield image

Topoisomerase II alpha Rabbit Polyclonal Antibody

Application: WB,IF-Cell,IHC-P,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated