Transferrin Mouse Monoclonal Antibody [E4-E6]
Catalog# M1510-17
Transferrin Mouse Monoclonal Antibody [E4-E6]
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IF-Cell
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IHC-P
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FC
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WB
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Human
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unconjugated
Overview
Product Name
Transferrin Mouse Monoclonal Antibody [E4-E6]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
Native protein.
Species Reactivity
Human
Validated Applications
IF-Cell, IHC-P, FC, WB
Molecular Weight
Predicted band size: 77 kDa
Positive Control
Human plasma, human serum, H22, HepG2, rat uterus tissue, human liver tissue, human kidney tissue.
Conjugation
unconjugated
Clone Number
E4-E6
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein G affinity purified.
Application Dilution
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
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WB
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1:1,000
Target
Function
Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Background References
1. Knisely A S et al. Molecular characterization of a third case of human atransferrinemia. Blood 104:2607-2607 (2004).
2. McGillivray R.T.A et al. The primary structure of human serum transferrin. The structures of seven cyanogen bromide fragments and the assembly of the complete structure. J Biol Chem 258:3543-3553 (1983).
Sequence Similarity
Belongs to the transferrin family.
Tissue Specificity
Expressed by the liver and secreted in plasma.
Subcellular Location
Secreted.
UNIPROT
Synonyms
Apotransferrin antibody
Beta 1 metal binding globulin antibody
Beta-1 metal-binding globulin antibody
DKFZp781D0156 antibody
PRO1400 antibody
PRO1557 antibody
PRO2086 antibody
Serotransferrin antibody
Serotransferrin precursor antibody
Siderophilin antibody
ExpandImages
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Western blot analysis of Transferrin on human plasma with Mouse anti-Transferrin antibody (M1510-17) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of Transferrin on human serum with Mouse anti-Transferrin antibody (M1510-17) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 77 kDa
Observed band size: 77 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1510-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. -
ICC staining Transferrin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Transferrin monoclonal antibody at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
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Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Transferrin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (M1510-17) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-Transferrin antibody (M1510-17) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (M1510-17) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Transferrin was done on HepG2 cells. The cells were fixed, permeabilized and stained with Transferrin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Reactivity: Human
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Reactivity: Human
Conjugate: unconjugated
