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VEGF Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# ER30607

VEGF Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

VEGF Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within N-terminal human VEGFA (P15692-1).

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P, FC

Molecular Weight

Predicted band size: 27 kDa

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, MCF7 cell lysate, U-87 MG cell lysate, K-562 cell lysate, HepG2, HUVEC, human lung carcinoma tissue, Hela.

Conjugation

unconjugated

RRID

AB_3069553

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

Target

Function

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. NRP1/Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. Isoform VEGF165B binds to KDR but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development.

Background References

1. "Vascular endothelial growth factor induces cyclooxygenase-dependent proliferation of endothelial cells via the VEGF-2 receptor." Murphy J.F., Fitzgerald D.J. FASEB J. 15:1667-1669(2001)

2. "A common polymorphism in the 5\'-untranslated region of the VEGF gene is associated with diabetic retinopathy in type 2 diabetes." Awata T., Inoue K., Kurihara S., Ohkubo T., Watanabe M., Inukai K., Inoue I., Katayama S. Diabetes 51:1635-1639(2002)

Sequence Similarity

Belongs to the PDGF/VEGF growth factor family.

Tissue Specificity

Isoform VEGF189, isoform VEGF165 and isoform VEGF121 are widely expressed. Isoform VEGF206 and isoform VEGF145 are not widely expressed. A higher level expression seen in pituitary tumors as compared to the pituitary gland.

Subcellular Location

Secreted.

UNIPROT

SwissProt: P15692 Human

Synonyms

Folliculostellate cell-derived growth factor antibody

Glioma-derived endothelial cell mitogen antibody

MGC70609 antibody

MVCD1 antibody

Vascular endothelial growth factor A antibody

vascular endothelial growth factor A121 antibody

vascular endothelial growth factor A165 antibody

vascular endothelial growth factor antibody

Vascular permeability factor antibody

VEGF A antibody

Expand

Images

  • Western blot analysis of VEGF on different lysates with Rabbit anti-VEGF antibody (<a href="/products/ER30607" style="font-weight: bold;text-decoration: underline;">ER30607</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: SH-SY5Y cell lysate<br />Lane 3: HUVEC cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: U-87 MG cell lysate<br />Lane 6: K-562 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 27 kDa<br /><br />Exposure time: 39 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER30607" style="font-weight: bold;text-decoration: underline;">ER30607</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of VEGF on different lysates with Rabbit anti-VEGF antibody (ER30607) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: HUVEC cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: U-87 MG cell lysate
    Lane 6: K-562 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 27 kDa

    Exposure time: 39 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER30607) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />All lanes: Western blot analysis of VEGF with anti-VEGF antibody (<a href="/products/ER30607" style="font-weight: bold;text-decoration: underline;">ER30607</a>) at 1:500 dilution.<br />Lane 1: Wild-type Hela whole cell lysate (10 µg).<br />Lane 2/3: VEGF knockdown Hela whole cell lysate (10 µg).<br /><br /><a href="/products/ER30607" style="font-weight: bold;text-decoration: underline;">ER30607</a> was shown to specifically react with VEGF in wild-type Hela cells. Weakened bands were observed when VEGF knockdown samples were tested. Wild-type and VEGF knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ER30607" style="font-weight: bold;text-decoration: underline;">ER30607</a>, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    All lanes: Western blot analysis of VEGF with anti-VEGF antibody (ER30607) at 1:500 dilution.
    Lane 1: Wild-type Hela whole cell lysate (10 µg).
    Lane 2/3: VEGF knockdown Hela whole cell lysate (10 µg).

    ER30607 was shown to specifically react with VEGF in wild-type Hela cells. Weakened bands were observed when VEGF knockdown samples were tested. Wild-type and VEGF knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER30607, 1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining VEGF in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • ICC staining VEGF in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    ICC staining VEGF in HUVEC cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

  • Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-VEGF antibody. Counter stained with hematoxylin.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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