Human/Monkey/Canine VEGF enzyme-linked immunoassay kit

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Catalog# EH0075

Human/Monkey/Canine VEGF enzyme-linked immunoassay kit

Application

ELISA

Reactivity

Human

Standard curve range

31.25~2000 pg/ml

Conjugation

unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Human/Monkey/Canine VEGF enzyme-linked immunoassay kit

Product Type

Elisa Kit

Sensitivity

1.04 pg/ml (50 μl)；3.21 pg/ml (10 μl)

pg/ml	O.D.		Average	Corrected
0.00	0.0275	0.0264	0.0270
31.25	0.1190	0.1082	0.1136	0.0867
62.50	0.1991	0.2040	0.2016	0.1746
125.00	0.3159	0.2863	0.3011	0.2742
250.00	0.5521	0.6045	0.5783	0.5514
500.00	1.1560	1.2260	1.1910	1.1641
1000.00	2.3170	2.2130	2.2650	2.2381
2000.00	3.6280	3.4990	3.5635	3.5366

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average (pg/ml)	53.2	229.1	694.5	31.6	160.6	438.1
Standard Deviation	3.6	13.3	38.4	2.1	8.2	13.6
Coefficient of Variation (%)	6.8	5.8	5.5	6.5	5.1	3.1

板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。

板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。

Spike Recovery

健康人血清加入3个不同浓度水平的人VEGF/VEGFA，未加入人VEGF/VEGFA的血清作为本底，计算回收率。
回收率的范围从94%至119%，平均回收率为105%。

Sample Value

Sample Matrix	Evaluated Sample	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	25.21-450.32	100	185.52

应用本试剂盒，检测30份健康人、2份健康猴和5份健康狗的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。

Experiment Type

Sandwich Elisa

Species Reactivity

Human

Detection Method

Colorimetric

Operation Duration

120min

Standard Curve Gradient

7点

Incubation Count

Detectable Sample

Dosing Volume

50 μl/10 μl

Product Features

Storage Condition

Store at 2-8 ℃

Application in Publications

ELISA

See 2 publications below

Application in Publications

Human

See 2 publications below

Target

UNIPROT

SwissProt: P15692 Human

Alias

Folliculostellate cell-derived growth factor

Glioma-derived endothelial cell mitogen

MGC70609

MVCD1

Vascular endothelial growth factor A

vascular endothelial growth factor A121

vascular endothelial growth factor A165

vascular endothelial growth factor

Vascular permeability factor

VEGF A

Expand

Function

血管内皮生长因子（VEGF或VEGF-A），也称为血管通透因子（VPF），是胎儿和成人血管生成和血管生成的有效介质。它是PDGF家族的成员，其特征在于胱氨酸结结构中存在八个保守的半胱氨酸残基并形成抗平行二硫键连接的二聚体。人类表达长度为 121、145、165、183、189 和 206 个氨基酸（aa）的交替剪接亚型。VEGF165似乎是最丰富和最有效的亚型，其次是VEGF121和VEGF189。VEGF121以外的亚型含有碱性肝素结合区，不能自由扩散。人VEGF165与小鼠和大鼠VEGF的相应区域共享88%的aa序列同一性。VEGF在多种细胞和组织中表达，包括骨骼肌和心肌、肝细胞、成骨细胞、中性粒细胞、巨噬细胞、角质形成细胞、棕色脂肪组织、CD34+干细胞、内皮细胞、成纤维细胞和血管平滑肌细胞。VEGF 表达由缺氧和细胞因子（如 IL-1、IL-6、IL-8、oncostatin M 和 TNF-α）诱导。VEGF亚型在发育过程中和成人中表达差异。 VEGF二聚体与两种相关的受体酪氨酸激酶VEGF R1（也称为Flt-1）和VEGF R2（Flk-1 / KDR）结合，并诱导其同源二聚化和自磷酸化。这些受体具有七个细胞外免疫球蛋白样结构域和一个细胞内分裂酪氨酸激酶结构域。它们在血管内皮细胞和一系列非内皮细胞上表达。尽管与 VEGF R1 结合的 VEGF 亲和力最高，但 VEGF R2 似乎是 VEGF 血管生成活性的主要介质。VEGF165还结合信号素受体神经皮林-1，后者促进与VEGF R2的复合物形成。 VEGF以其在血管生成中的作用而闻名。在胚胎发生过程中，VEGF调节内皮细胞的增殖，迁移和存活，从而调节血管密度和大小，但在决定血管模式方面没有作用。VEGF通过成骨细胞和软骨细胞募集促进骨形成，也是一种单核细胞化学引诱剂。出生后，VEGF维持内皮细胞的完整性，是微血管和大血管内皮细胞的有效丝裂原。在成人中，VEGF主要在伤口愈合和女性生殖周期中起作用。在患病组织中，VEGF促进血管通透性。因此，它被认为通过促进外渗和肿瘤血管生成来促进肿瘤转移。已经采用了各种治疗策略来拮抗VEGF介导的肿瘤血管生成。循环 VEGF 水平与自身免疫性疾病（如类风湿性关节炎、多发性硬化症和系统性红斑狼疮）的疾病活动度相关。

Citation

Filter by Application

Filter by Reactivity

Carboxymethyl chitosan hydrogel reinforced by endothelial cell derivatives for angiogenesis and full-thickness wound healing
- Journal: Materials & Design
- DOI: 10.1016/j.matdes.2025.115242
- IF: 7.9
- Application: ELISA
- Reactivity: Human
- Publish date: 2025 Nov
A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
- Journal: Bioactive Materials
- DOI: 10.1016/j.bioactmat.2024.11.005
- IF: 18
- Application: ELISA
- Reactivity: Human
- Publish date: 2024 Dec

Human/Monkey/Canine VEGF enzyme-linked immunoassay kit

Usd:

Overview

Product Name

Product Type

Sensitivity

Precision

Spike Recovery

Sample Value

Experiment Type

Species Reactivity

Detection Method

Operation Duration

Standard Curve Gradient

Incubation Count

Detectable Sample

Dosing Volume

Product Features

Storage Condition

Application in Publications

Application in Publications

Target

UNIPROT

Alias

Function

Citation

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