Human/Monkey/Canine EGF enzyme-linked immunoassay kit
Catalog# EH0023
Human/Monkey/Canine EGF enzyme-linked immunoassay kit
Application
-
ELISA
Reactivity
-
Human
Standard curve range
-
1.37~1000 pg/ml
Conjugation
-
unconjugated
Overview
Product Name
Human/Monkey/Canine EGF enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.12 pg/ml (50 μl)；0.59 pg/ml (10 μl)
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0115 | 0.0121 | 0.0118 | |
| 1.37 | 0.0293 | 0.0297 | 0.0295 | 0.0177 |
| 4.12 | 0.0676 | 0.0683 | 0.0680 | 0.0562 |
| 12.35 | 0.1633 | 0.1675 | 0.1654 | 0.1536 |
| 37.04 | 0.4536 | 0.4552 | 0.4544 | 0.4426 |
| 111.11 | 1.1453 | 1.1946 | 1.1700 | 1.1582 |
| 333.33 | 2.3065 | 2.4491 | 2.3778 | 2.3660 |
| 1000.00 | 3.2412 | 3.2825 | 3.2619 | 3.2501 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 13.2 | 61.3 | 217.8 | 20.4 | 105.0 | 347.9 |
| Standard Deviation | 0.5 | 4.1 | 6.6 | 1.0 | 5.3 | 19.3 |
| Coefficient of Variation (%) | 3.5 | 6.7 | 3.0 | 4.9 | 5.1 | 5.6 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人EGF，未加人 EGF 的血清作为本底，计算回收率。回收率的范围从 78 % 至 106 %，平均回收率为 93 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 200.7-417.0 | 100 | 304.2 |
应用本试剂盒，检测30份健康人、2份健康猴和5份健康狗的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Sandwich Elisa
Species Reactivity
Human
Detection Method
Colorimetric
Operation Duration
120min
Standard Curve Gradient
7点/3倍
Incubation Count
2
Detectable Sample
Dosing Volume
50 μl/10 μl
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Beta urogastrone
beta-urogastrone
EGF
EGF_HUMAN
Epidermal growth factor
HOMG4
OTTHUMP00000219721
OTTHUMP00000219722
Pro epidermal growth factor
URG
ExpandFunction
EGF（表皮生长因子）表示为跨膜蛋白，经蛋白水解裂解以产生可溶性形式。它通过EGF R / ErbB1发出信号，EGF R / ErbB1与ErbB2，ErbB3或ErbB4异源二聚体，也可以与PDGF R beta和HGF R / c-MET结合。在发育过程中，EGF调节胸腺细胞分化，神经胶质细胞产生和脂肪细胞成熟。在成人中，EGF 在乳腺泌乳、成纤维细胞有丝分裂、细胞外基质解离和细胞迁移中发挥作用。