Human/Monkey/Canine NCAM-1/CD56 enzyme-linked immunoassay kit
Catalog# EH0102
Human/Monkey/Canine NCAM-1/CD56 enzyme-linked immunoassay kit
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ELISA
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Human
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0.03~20 ng/ml
Overview
Product Name
Human/Monkey/Canine NCAM-1/CD56 enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
3.69 pg/ml (50 μl)；37.5 pg/ml (10 μl)
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0268 | 0.0287 | 0.0278 | |
| 0.03 | 0.0416 | 0.0360 | 0.0388 | 0.0111 |
| 0.08 | 0.0543 | 0.0660 | 0.0602 | 0.0324 |
| 0.25 | 0.1195 | 0.1114 | 0.1155 | 0.0877 |
| 0.74 | 0.2833 | 0.2774 | 0.2804 | 0.2526 |
| 2.22 | 0.6849 | 0.7219 | 0.7034 | 0.6757 |
| 6.67 | 1.9060 | 1.9530 | 1.9295 | 1.9018 |
| 20.00 | 3.7930 | 3.9828 | 3.8879 | 3.8602 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 233.5 | 1498.4 | 4930.0 | 366.5 | 1837.9 | 5636.7 |
| Standard Deviation | 18.1 | 103.1 | 326.6 | 10.0 | 52.2 | 150.9 |
| Coefficient of Variation (%) | 7.8 | 6.9 | 6.6 | 2.7 | 2.8 | 2.7 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 NCAM-1/CD56，未加人 NCAM-1/CD56 的血清作为本底，计算回收率。回收率的范围从 80 % 至 98 %，平均回收率为 88 %。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 286.3-459.6 | 100 | 370.6 |
应用本试剂盒，检测30份健康人、2份健康猴和5份健康狗的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
antigen MSK39 identified by monoclonal
5.1H11
antigen recognized by monoclonal
5.1H11
CD56
cell adhesion molecule, neural, 1
MSK 39
MSK39
N-CAM-1
NCAM 1
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