Human/Monkey/Canine IL-1β enzyme-linked immunoassay kit
Catalog# EH0006
Human/Monkey/Canine IL-1β enzyme-linked immunoassay kit
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ELISA
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Human
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3.91~250 pg/ml
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unconjugated
Overview
Product Name
Human/Monkey/Canine IL-1β enzyme-linked immunoassay kit
Product Type
Elisa Kit
Sensitivity
0.18 pg/ml (10 μl)；0.09 pg/ml (50 μl)；
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0296 | 0.0307 | 0.0302 | |
| 3.91 | 0.1330 | 0.1232 | 0.1281 | 0.0980 |
| 7.81 | 0.2132 | 0.2122 | 0.2127 | 0.1826 |
| 15.63 | 0.3820 | 0.3760 | 0.3790 | 0.3489 |
| 31.25 | 0.7406 | 0.6980 | 0.7193 | 0.6892 |
| 62.50 | 1.3710 | 1.2880 | 1.3295 | 1.2994 |
| 125.00 | 2.4490 | 2.3060 | 2.3775 | 2.3474 |
| 250.00 | 3.7620 | 3.6230 | 3.6925 | 3.6624 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (pg/ml) | 6.0 | 29.6 | 93.7 | 7.2 | 33.6 | 104.4 |
| Standard Deviation | 0.3 | 1.3 | 4.1 | 0.3 | 1.0 | 2.0 |
| Coefficient of Variation (%) | 4.8 | 4.5 | 4.4 | 4.1 | 2.9 | 1.9 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-1 beta into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 85% to 120% with an overall mean recovery of 95%.
Sample Value
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Human Serum | 30 | 1.56-60.87 | 100 | 15.59 |
| Monkey Serum | 2 | 48.96-51.40 | 100 | 50.18 |
| Canine Serum | 5 | 12.00-16.03 | 100 | 14.42 |
Serum/Plasma – Thirty samples from apparently healthy volunteers, two samples from healthy monkeys and five samples from healthy canines were evaluated for the presence of human IL-1 beta in this assay. No medical histories were available for the donors.
n.d. = non-detectable. Samples measured below the sensitivity are considered to be non-detectable.
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Application in Publications
| ELISA | See 4 publications below |
Application in Publications
| Human | See 4 publications below |
Target
UNIPROT
Alias
Function
Citation
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HK2-driven histone H3K18 lactylation promotes stromal cell senescence and decidualization deficiency in URSA via CUX1-mediated SASP factor transcription
Journal: Cellular & Molecular Biology Letters
DOI: 10.1186/s11658-026-00879-y
IF: 10.2
Application: ELISA
Reactivity: Human
Publish date: 2026 Mar
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A high-throughput reporter assay for screening potential gasdermin D inhibitors from natural products
Journal: International Journal Of Biological Macromolecules
DOI: 10.1016/j.ijbiomac.2026.150110
IF: 8.5
Application: ELISA
Reactivity: Human
Publish date: 2026 Jan
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Inhibition of the α7 nicotinic acetylcholine receptor alleviates pyroptosis in blood-brain barrier induced by Cryptococcus neoformans
Journal: Microbial Pathogenesis
DOI: 10.1016/j.micpath.2026.108377
IF: 3.5
Application: ELISA
Reactivity: Human
Publish date: 2026 Feb
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A ceramic microbridge microfluidic chip to study osteogenic differentiation of mesenchymal stem cells in bioactive ceramic immune microenvironment
Journal: Bioactive Materials
DOI: 10.1016/j.bioactmat.2024.11.005
IF: 18
Application: ELISA
Reactivity: Human
Publish date: 2024 Dec