Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)
Catalog# EHY0027
Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)
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ELISA
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Human
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0.03~20 ng/ml
Overview
Product Name
Human/Monkey/Canine CCL5/RANTES enzyme-linked immunoassay kit(one step)
Product Type
Elisa Kit
Sensitivity
0.7 pg/ml (50 μl)；4.99 pg/ml (10 μl)；
| ng/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0.00 | 0.0118 | 0.0111 | 0.0115 | |
| 0.03 | 0.0329 | 0.0351 | 0.0340 | 0.0226 |
| 0.08 | 0.0712 | 0.0721 | 0.0717 | 0.0602 |
| 0.25 | 0.1790 | 0.1795 | 0.1793 | 0.1678 |
| 0.74 | 0.4966 | 0.5132 | 0.5049 | 0.4935 |
| 2.22 | 1.3920 | 1.4070 | 1.3995 | 1.3881 |
| 6.67 | 3.5510 | 3.3680 | 3.4595 | 3.4481 |
| 20.00 | 4.2729 | 4.4325 | 4.3527 | 4.3413 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average (ng/ml) | 0.3 | 1.5 | 6.6 | 0.3 | 1.5 | 6.7 |
| Standard Deviation | 0.0 | 0.1 | 0.3 | 0.0 | 0.1 | 0.4 |
| Coefficient of Variation (%) | 5.0 | 3.4 | 4.9 | 5.1 | 5.6 | 5.4 |
板内精密度：3 个已知浓度的样本酶标板内重复测定 20 次，评估酶标板内的精密度。
板间精密度：3 个已知浓度的样本酶标板间重复检测 6 次，评估酶标板间的精密度。
Spike Recovery
健康人血清加入 3 个不同浓度水平的人 CCL5/RANTES，未加人 CCL5/RANTES 的血清作为本底，计算回收率。回收率的范围从 73 % 至 124 %，平均回收率为 97%。
Sample Value
| Sample Matrix | Evaluated Sample | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 5.88-31.87 | 100 | 19.53 |
应用本试剂盒，检测30份健康人、2份健康猴和5份健康狗的血清样本。n.d. = 测不到浓度值。样本的浓度值低于灵敏度被认为测不到浓度值。
Experiment Type
Species Reactivity
Detection Method
Operation Duration
Standard Curve Gradient
Incubation Count
2
Detectable Sample
Dosing Volume
Product Features
Storage Condition
Store at 2-8 ℃
Target
UNIPROT
Alias
Beta chemokine RANTES
Beta chemokine RANTES precursor
C C motif chemokine 5
CCL 5
CCL5
CCL5_HUMAN
Chemokine (C C motif) ligand 5
Chemokine CC Motif Ligand 5
D17S136E
EoCP
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