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ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750788

ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human ZIP Kinase aa 250-454 (O43293).

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 53 kDa

Positive Control

A431 cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, Caco-2 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, human kidney tissue lysate.

Conjugation

unconjugated

Clone Number

PSH02-18

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

Target

Function

ZIP Kinase is a serine/threonine kinase, which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.

Background References

1. Kocher BA et al. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res 13:358-67 (2015).

2. Marina O et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res 70:1344-55 (2010).

Subcellular Location

Nucleus, Cytoplasm

UNIPROT

SwissProt: O43293 Human

Synonyms

DAP kinase 3 antibody

DAP like kinase antibody

DAP-like kinase antibody

Dapk 3 antibody

DAPK3 antibody

DAPK3_HUMAN antibody

Death associated kinase 3 antibody

Death associated protein kinase 3 antibody

Death-associated protein kinase 3 antibody

Dlk antibody

Expand

Images

  • Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (<a href="/products/HA750788" style="font-weight: bold;text-decoration: underline;">HA750788</a>) at 1/1,000 dilution.<br /><br />Lane 1: A431 cell lysate<br />Lane 2: A549 cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: SiHa cell lysate<br />Lane 5: HepG2 cell lysate<br />Lane 6: Hela cell lysate<br />Lane 7: Jurkat cell lysate<br />Lane 8: Caco-2 cell lysate<br />Lane 9: K-562 cell lysate<br />Lane 10: MCF7 cell lysate<br />Lane 11: U-2 OS cell lysate<br />Lane 12: Human kidney tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750788" style="font-weight: bold;text-decoration: underline;">HA750788</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA750788) at 1/1,000 dilution.

    Lane 1: A431 cell lysate
    Lane 2: A549 cell lysate
    Lane 3: 293T cell lysate
    Lane 4: SiHa cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: Hela cell lysate
    Lane 7: Jurkat cell lysate
    Lane 8: Caco-2 cell lysate
    Lane 9: K-562 cell lysate
    Lane 10: MCF7 cell lysate
    Lane 11: U-2 OS cell lysate
    Lane 12: Human kidney tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 50 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (<a href="/products/HA750788" style="font-weight: bold;text-decoration: underline;">HA750788</a>) at 1/2,000 dilution.<br /><br />Lane 1: HCT 116-si NT cell lysate<br />Lane 2: HCT 116-si ZIP Kinase cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 53 kDa<br />Observed band size: 53 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750788" style="font-weight: bold;text-decoration: underline;">HA750788</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA750788) at 1/2,000 dilution.

    Lane 1: HCT 116-si NT cell lysate
    Lane 2: HCT 116-si ZIP Kinase cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750788) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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metafield image

ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]

Application: WB

Reactivity: Human

Conjugate: unconjugated