ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]
Catalog# HA721777
ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]
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WB
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Human
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unconjugated
Overview
Product Name
ZIP Kinase Recombinant Rabbit Monoclonal Antibody [PSH02-18]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ZIP Kinase aa 250-454 (O43293).
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 53 kDa
Positive Control
A431 cell lysate, A549 cell lysate, 293T cell lysate, SiHa cell lysate, HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, Caco-2 cell lysate, K-562 cell lysate, MCF7 cell lysate, U-2 OS cell lysate, human kidney tissue lysate.
Conjugation
unconjugated
Clone Number
PSH02-18
RRID
Reactivity Data
Verified (internally validated)
Reported in literature (not internally validated)
Predicted reactive (based on sequence homology)
Not recommended (failed internal validation)
| WB | IHC-P | FC | IF-Cell | |
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| mouse |
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| rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000-1:2,000
Target
Function
ZIP Kinase is a serine/threonine kinase, which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
Background References
1. Kocher BA et al. DAPK3 suppresses acini morphogenesis and is required for mouse development. Mol Cancer Res 13:358-67 (2015).
2. Marina O et al. Serologic markers of effective tumor immunity against chronic lymphocytic leukemia include nonmutated B-cell antigens. Cancer Res 70:1344-55 (2010).
Subcellular Location
Nucleus, Cytoplasm
UNIPROT
Synonyms
DAP kinase 3 antibody
DAP like kinase antibody
DAP-like kinase antibody
Dapk 3 antibody
DAPK3 antibody
DAPK3_HUMAN antibody
Death associated kinase 3 antibody
Death associated protein kinase 3 antibody
Death-associated protein kinase 3 antibody
Dlk antibody
ExpandImages
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Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: A549 cell lysate
Lane 3: 293T cell lysate
Lane 4: SiHa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: Hela cell lysate
Lane 7: Jurkat cell lysate
Lane 8: Caco-2 cell lysate
Lane 9: K-562 cell lysate
Lane 10: MCF7 cell lysate
Lane 11: U-2 OS cell lysate
Lane 12: Human kidney tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of ZIP Kinase on different lysates with Rabbit anti-ZIP Kinase antibody (HA721777) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si ZIP Kinase cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721777) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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