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Bcl-2 Rabbit Polyclonal Antibody (ER1802-97)

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Catalog# ER1802-97

Rabbit Polyclonal to Bcl-2

Tested for

  • WB

  • IF-Cell

  • FC

Reacts with

  • Human

  • Mouse

This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Rabbit polyclonal primary

Product Name

Bcl-2 Rabbit Polyclonal Antibody (ER1802-97)

Immunogen

Synthetic peptide within human bcl-2 20-100 aa.

Host

Rabbit

Positive Control

HL-60, mouse colon tissue lysate, LOVO, MCF-7, SH-SY-5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3069197

APPLICATION DILUTION

  • WB

  • 1:200-1:500

  • IF-Cell

  • 1:50-1:200

  • FC

  • 1:50-1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Immunogen affinity purified.

Molecular Weight

22/26 kDa

Isotype

IgG

TARGET

UNIPROT #

P10415

PROTEIN NAME

Bcl-2

SYNONYMS

Apoptosis regulator Bcl 2 antibody;Apoptosis regulator Bcl-2 antibody;Apoptosis regulator Bcl2 antibody;AW986256 antibody;B cell CLL/lymphoma 2 antibody;B cell leukemia/lymphoma 2 antibody;Bcl-2 antibody;Bcl2 antibody;BCL2_HUMAN antibody;C430015F12Rik antibody;D630044D05Rik antibody;D830018M01Rik antibody;Leukemia/lymphoma, B-cell, 2 antibody;Oncogene B-cell leukemia 2 antibody;PPP1R50 antibody;Protein phosphatase 1, regulatory subunit 50 antibody;Bcl 2 antibody

SEQUENCE SIMILARITIES

Belongs to the Bcl-2 family.

TISSUE SPECIFICITY

Expressed in a variety of tissues.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity).; Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.; Monoubiquitinated by PRKN, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome.

SUBCELLULAR LOCATION

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm.

FUNCTION

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release.

CITATIONS

  • Rui Zhang,et al

    DNA hypomethylation of Syk induces oxidative stressand apoptosis via the PKCβ/P66shc signaling pathway indiabetic kidney disease

  • Deng, Y. L., Chen, S., Wang, H. T., Wang, B., & Xiao, K.

    Prescription of Sageretia hamosa Brongn Relieved Goiter through Promoted Apoptosis of Thyroid Cells via miR-511-3p and PTEN/PI3K/Akt Pathway

  • He, Y., Zhao, T., Chen, F., Song, C., Zhong, C., & Luo, Z.

    Functional Analysis of the Promoter Regions of Two Apoptosis-Related Genes (Bcl-2 and Cycs) and Their Regulation by Zn in Yellow Catfish. International Journal of Molecular Sciences, 22(12), 6291.

  • Ye Xiaohang,et al

    Human Amniotic Epithelial Stem Cells Alleviate Autoimmune Premature Ovarian Insufficiency in Mice by Targeting Granulosa Cells via AKT/ERK Pathways

  • Yanli Zhang,et al

    Pyrroloquinoline Quinone Alleviates Mitochondria Damage in Radiation-Induced Lung Injury in a MOTS-c-Dependent Manner

  • Wang, F., Liu, Y., Ni, F., Jin, J., Wu, Y., Huang, Y., Ye, X., Shen, X., Ying, Y., Chen, J., Chen, R., Zhang, Y., Sun, X., Wang, S., Xu, X., Chen, C., Guo, J., & Zhang, D.

    BNC1 deficiency-triggered ferroptosis through the NF2-YAP pathway induces primary ovarian insufficiency

  • Wang, C., Wei, S., Liu, B., Wang, F., Lu, Z., Jin, M., & Wang, Y.

    Maternal consumption of a fermented diet protects offspring against intestinal inflammation by regulating the gut microbiota

  • Zhang Yanli,et al

    The Mitochondrial-Derived Peptide MOTS-c Alleviates Radiation Pneumonitis via an Nrf2-Dependent Mechanism

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