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beta Tubulin Mouse Monoclonal Antibody [1-B11]

Usd: 350

Specification

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Catalog# EM0103

beta Tubulin Mouse Monoclonal Antibody [1-B11]

Application

  • WB

  • IF-Cell

  • IHC-P

  • FC

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

  • Zebrafish

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

beta Tubulin Mouse Monoclonal Antibody [1-B11]

Antibody Type

Mouse Monoclonal Antibody

Immunogen

Synthetic peptide within human Beta tubulin aa 51-100.

Species Reactivity

Human, Mouse, Rat, Zebrafish

Validated Applications

WB, IF-Cell, IHC-P, FC, IF-Tissue

Molecular Weight

Predicted band size: 50 kDa

Positive Control

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, HeLa, NIH/3T3, PC-12, hybrid fish (crucian-carp) brain tissue lysates, human colon cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, Rat kidney tissue lysate, Rat heart tissue lysate, Rat liver tissue lysate.

Conjugation

unconjugated

Clone Number

1-B11

RRID

AB_2819165

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:20,000-1:50,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:3,000-1:10,000

  • IF-Tissue

  • 1:500-1:2,000

Target

Function

Tubulin in molecular biology can refer either to the tubulin protein superfamily of globular proteins, or one of the member proteins of that superfamily. α- and β-tubulins polymerize into microtubules, a major component of the eukaryotic cytoskeleton. Microtubules function in many essential cellular processes, including mitosis. Tubulin-binding drugs kill cancerous cells by inhibiting microtubule dynamics, which are required for DNA segregation and therefore cell division. In eukaryotes, there are six members of the tubulin superfamily, although not all are present in all species. Both α and β tubulins have a mass of around 50 kDa and are thus in a similar range compared to actin (with a mass of ~42 kDa). In contrast, tubulin polymers (microtubules) tend to be much bigger than actin filaments due to their cylindrical nature. Tubulin was long thought to be specific to eukaryotes. More recently, however, several prokaryotic proteins have been shown to be related to tubulin.

Background References

1. "Evolutionary history of a multigene family: an expressed human beta-tubulin gene and three processed pseudogenes." Lee M.G.-S., Lewis S.A., Wilde C.D., Cowan N.J. Cell 33:477-487(1982)

2. "Tubulins in the primate retina: evidence that xanthophylls may be endogenous ligands for the paclitaxel-binding site." Crabtree D.V., Ojima I., Geng X., Adler A.J. Bioorg. Med. Chem. 9:1967-1976(2000)

3. "Mutations in the beta-tubulin gene TUBB5 cause microcephaly with structural brain abnormalities." Breuss M., Heng J.I., Poirier K., Tian G., Jaglin X.H., Qu Z., Braun A., Gstrein T., Ngo L., Haas M., Bahi-Buisson N., Moutard M.L., Passemard S., Verloes A., Gressens P., Xie Y., Robson K.J., Rani D.S. Cell Rep. 2:1554-1562(2011)

Sequence Similarity

Belongs to the tubulin family.

Tissue Specificity

Ubiquitously expressed with highest levels in spleen, thymus and immature brain.

Post-translational Modification

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold.; Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).; Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

Subcellular Location

Cytoplasm ,cytoskeleton

UNIPROT

SwissProt: P07437 Human

SwissProt: P99024 Mouse

SwissProt: P69897 Rat

Synonyms

Beta 4 tubulin antibody

Beta 5 tubulin antibody

BetaTubulin antibody

TBB5_HUMAN antibody

TUBB antibody

TUBB2 antibody

TUBB2A antibody

TUBB5 antibody

tubulin beta 2A antibody

Tubulin beta chain antibody

Expand

Images

  • Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/20,000 dilution.<br /><br />Lane 1: K-562 cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: NIH/3T3 cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: C6 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute 40 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1:50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (EM0103) at 1/20,000 dilution.

    Lane 1: K-562 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: NIH/3T3 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: C6 cell lysate

    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute 40 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0103) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NIH/3T3 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Immunocytochemistry analysis of PC-12 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />beta Tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 594, <a href="/products/HA1122" style="font-weight: bold;text-decoration: underline;">HA1122</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling beta Tubulin with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-beta Tubulin antibody (EM0103) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

  • Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/5,000 dilution.

    Western blot analysis of β-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-β-tubulin antibody at 1/5,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-beta Tubulin antibody (EM0103) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0103) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/3,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-beta Tubulin antibody (EM0103) at 1/3,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0103) at 1/3,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-beta Tubulin antibody (EM0103) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/10,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-beta Tubulin antibody (EM0103) at 1/10,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (EM0103) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/1,000 dilution.<br /><br />Lane 1: Rat kidney tissue lysate<br />Lane 2: Rat heart tissue lysate<br />Lane 3: Rat liver tissue lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 50 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 45 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/EM0103" style="font-weight: bold;text-decoration: underline;">EM0103</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of beta Tubulin on different lysates with Mouse anti-beta Tubulin antibody (EM0103) at 1/1,000 dilution.

    Lane 1: Rat kidney tissue lysate
    Lane 2: Rat heart tissue lysate
    Lane 3: Rat liver tissue lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 50 kDa
    Observed band size: 50 kDa

    Exposure time: 45 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM0103) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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