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c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610220

c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Application

  • IHC-Fr

  • WB

Reactivity

  • Mouse

  • Rat

  • Human

With BSA and Azide
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Predicted species support after-sales service
Conjugation

  • unconjugated

Overview

Product Name

c-Fos Recombinant Antibody [PSH05-77] - Guinea pig IgG2 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within human Protein c-Fos aa 1-380.

Product Specificity

This Guinea pig Recombinant monoclonal chimeric antibody has been engineered from a HiMab parent antibody (HA722666). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed FC-reactive secondary antibodies are recommended.

Species Reactivity

Mouse, Rat, Human (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

IHC-Fr, WB

Positive Control

Mouse brain tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

PSH05-77

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-Fr

  • 1:2,000-1:5,000

  • WB

  • 1:5,000-1:10,000

Target

Function

Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos. It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2. It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression. It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.

Background References

1. Matsuoka K et al. Metabolic rewiring controlled by c-Fos governs cartilage integrity in osteoarthritis. Ann Rheum Dis. 2023 Sep

2. Osada N et al. c-FOS is an integral component of the IKZF1 transactivator complex and mediates lenalidomide resistance in multiple myeloma. Clin Transl Med. 2023 Aug

Subcellular Location

Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.

UNIPROT

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Synonyms

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

Cellular oncogene fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ murine osteosarcoma viral oncogene homolog antibody

FBJ murine osteosarcoma viral v fos oncogene homolog antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Cerebral cortex (restraint stress induced)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/2,000 (c-Fos, <a href="/products/HA610220" style="font-weight: bold;text-decoration: underline;">HA610220</a>, Guinea pig, Red); 1/1,000 (Iba1, <a href="/products/ET1705-78" style="font-weight: bold;text-decoration: underline;">ET1705-78</a>, Rabbit, Green)<br /><br />Antigen retrieval: Not required<br /><br />Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

    Application: IHC-Fr

    Species: Mouse

    Site: Cerebral cortex (restraint stress induced)

    Sample: Frozen section

    Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green)

    Antigen retrieval: Not required

    Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

  • Application: IHC-Fr<br /><br />Species: Rat<br /><br />Site: Cerebral cortex (restraint stress induced)<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/2,000 (c-Fos, <a href="/products/HA610220" style="font-weight: bold;text-decoration: underline;">HA610220</a>, Guinea pig, Red); 1/1,000 (Iba1, <a href="/products/ET1705-78" style="font-weight: bold;text-decoration: underline;">ET1705-78</a>, Rabbit, Green)<br /><br />Antigen retrieval: Not required<br /><br />Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

    Application: IHC-Fr

    Species: Rat

    Site: Cerebral cortex (restraint stress induced)

    Sample: Frozen section

    Antibody concentration: 1/2,000 (c-Fos, HA610220, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green)

    Antigen retrieval: Not required

    Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (<a href="/products/HA610220" style="font-weight: bold;text-decoration: underline;">HA610220</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate<br />Lysates/proteins at 15 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41-55 kDa<br />Exposure time: 6 seconds; ECL: K1801;<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA610220" style="font-weight: bold;text-decoration: underline;">HA610220</a>) at 1/5,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (<a href="/products/HA1021" style="font-weight: bold;text-decoration: underline;">HA1021</a>) at 1/10,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (HA610220) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate
    Lysates/proteins at 15 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41-55 kDa
    Exposure time: 6 seconds; ECL: K1801;

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610220) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/10,000 dilution was used for 1 hour at room temperature.

  • Application: Immunofluorescence (IHC-Fr)<br /><br />Species: Mouse<br />Tissue: Cerebral cortex (restraint stress induced)<br />Sample: Frozen section<br /><br />Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature. <br /><br />Wash buffer: 1× TBST<br />Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.<br />Primary antibody: c-Fos, 1/5,000 (<a href="/products/HA610220" style="font-weight: bold;text-decoration: underline;">HA610220</a>, guinea pig, Green), Neurogranin, 1/500 (<a href="/products/HA721915" style="font-weight: bold;text-decoration: underline;">HA721915</a>, rabbit, Red), overnight at 4℃.<br />Secondary antibody: Goat Anti-Guinea pig IgG (iFluor&trade; 594, <a href="/products/HA1143" style="font-weight: bold;text-decoration: underline;">HA1143</a>), Goat anti-Rabbit IgG (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IHC-Fr)

    Species: Mouse
    Tissue: Cerebral cortex (restraint stress induced)
    Sample: Frozen section

    Antigen retrieval: 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 0.5 % Triton X-100 + 0.3 M Glycine in PBS, 10 minutes at room temperature.
    Primary antibody: c-Fos, 1/5,000 (HA610220, guinea pig, Green), Neurogranin, 1/500 (HA721915, rabbit, Red), overnight at 4℃.
    Secondary antibody: Goat Anti-Guinea pig IgG (iFluor™ 594, HA1143), Goat anti-Rabbit IgG (iFluor™ 488, HA1121), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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