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c-Fos Recombinant Rabbit Monoclonal Antibody [JJ0938]

Usd: 385

Specification

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Catalog# ET1701-95

c-Fos Recombinant Rabbit Monoclonal Antibody [JJ0938]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

c-Fos Recombinant Rabbit Monoclonal Antibody [JJ0938]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human c-Fos aa 231-268 / 380.

Species Reactivity

Human, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 41 kDa

Positive Control

HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cell lysate, PC-12 serum starved for 16 hours then treated with 200nM TSA for 4 hours cell lysate, HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours, human placenta tissue.

Conjugation

unconjugated

Clone Number

JJ0938

RRID

AB_3070261

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:50-1:200

Target

Function

Protein c-Fos is a proto-oncogene that is the human homolog of the retroviral oncogene v-fos.[5] It is encoded in humans by the FOS gene. It was first discovered in rat fibroblasts as the transforming gene of the FBJ MSV (Finkel–Biskis–Jinkins murine osteogenic sarcoma virus) (Curran and Tech, 1982). It is a part of a bigger Fos family of transcription factors which includes c-Fos, FosB, Fra-1 and Fra-2.It has been mapped to chromosome region 14q21→q31. c-Fos encodes a 62 kDa protein, which forms heterodimer with c-jun (part of Jun family of transcription factors), resulting in the formation of AP-1 (Activator Protein-1) complex which binds DNA at AP-1 specific sites at the promoter and enhancer regions of target genes and converts extracellular signals into changes of gene expression.It plays an important role in many cellular functions and has been found to be overexpressed in a variety of cancers.

Background References

1. Dantas-Ferreira, R.F. et al. 2015. Food-anticipatory activity in Syrian hamsters: behavioral and molecular responses in the hypothalamus according to photoperiodic conditions. PloS one. 10: e0126519.

2. Zhang, J. et al. 2015. Effect of BioAggregate on Receptor Activator of Nuclear Factor-Kappa B Ligand-induced Osteoclastogenesis from Murine Macrophage Cell Line In Vitro. Journal of endodontics. 41: 1265-71.

Sequence Similarity

Belongs to the bZIP family. Fos subfamily.

Post-translational Modification

Phosphorylated in the C-terminal upon stimulation by nerve growth factor (NGF) and epidermal growth factor (EGF). Phosphorylated, in vitro, by MAPK and RSK1. Phosphorylation on both Ser-362 and Ser-374 by MAPK1/2 and RSK1/2 leads to protein stabilization with phosphorylation on Ser-374 being the major site for protein stabilization on NGF stimulation. Phosphorylation on Ser-362 and Ser-374 primes further phosphorylations on Thr-325 and Thr-331 through promoting docking of MAPK to the DEF domain. Phosphorylation on Thr-232, induced by HA-RAS, activates the transcriptional activity and antagonizes sumoylation. Phosphorylation on Ser-362 by RSK2 in osteoblasts contributes to osteoblast transformation (By similarity).; Constitutively sumoylated with SUMO1, SUMO2 and SUMO3. Desumoylated by SENP2. Sumoylation requires heterodimerization with JUN and is enhanced by mitogen stimulation. Sumoylation inhibits the AP-1 transcriptional activity and is, itself, inhibited by Ras-activated phosphorylation on Thr-232.; In quiescent cells, the small amount of FOS present is phosphorylated at Tyr-10 and Tyr-30 by SRC. This Tyr-phosphorylated form is cytosolic. In growing cells, dephosphorylated by PTPN2. Dephosphorylation leads to the association with endoplasmic reticulum membranes and activation of phospholipid synthesis.

Subcellular Location

Nucleus, Endoplasmic reticulum, Cytoplasm.

UNIPROT

SwissProt: P01100 Human

SwissProt: P01101 Mouse

SwissProt: P12841 Rat

Synonyms

Activator protein 1 antibody

AP 1 antibody

C FOS antibody

Cellular oncogene c fos antibody

Cellular oncogene fos antibody

FBJ murine osteosarcoma viral (v fos) oncogene homolog (oncogene FOS) antibody

FBJ murine osteosarcoma viral oncogene homolog antibody

FBJ murine osteosarcoma viral v fos oncogene homolog antibody

FBJ Osteosarcoma Virus antibody

FOS antibody

Expand

Images

  • Western blot analysis of c-Fos on different lysates with Rabbit anti-c-Fos antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41/55 kDa<br /><br />Exposure time: 5 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of c-Fos on different lysates with Rabbit anti-c-Fos antibody (ET1701-95) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41/55 kDa

    Exposure time: 5 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of c-Fos on different lysates with Rabbit anti-c-Fos antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/1,000 dilution.<br /><br />Lane 1: PC-12 cell lysate<br />Lane 2: PC-12 serum starved for 16 hours then treated with 200nM TSA for 4 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 41 kDa<br />Observed band size: 41/55 kDa<br /><br />Exposure time: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of c-Fos on different lysates with Rabbit anti-c-Fos antibody (ET1701-95) at 1/1,000 dilution.

    Lane 1: PC-12 cell lysate
    Lane 2: PC-12 serum starved for 16 hours then treated with 200nM TSA for 4 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 41 kDa
    Observed band size: 41/55 kDa

    Exposure time: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-95) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunocytochemistry analysis of normal HeLa / HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cells labeling c-Fos with Rabbit anti-c-Fos antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    ☑ Cell treatment (CT)

    Immunocytochemistry analysis of normal HeLa / HeLa serum starved for 40 hours then treated with 20% FBS for 2 hours cells labeling c-Fos with Rabbit anti-c-Fos antibody (ET1701-95) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-c-Fos antibody (ET1701-95) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Fos antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/ET1701-95" style="font-weight: bold;text-decoration: underline;">ET1701-95</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-c-Fos antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-95, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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