cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71]

Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution.

Lane 1: HT-29 cell lysate (20 µg/Lane)
Lane 2: TF-1 cell lysate (20 µg/Lane)
Lane 3: HepG2 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: Jurkat cell lysate (20 µg/Lane)
Lane 7: A549 cell lysate (20 µg/Lane)
Lane 8: HEK-293 cell lysate (20 µg/Lane)
Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
Lane 10: PC-12 cell lysate (20 µg/Lane)
Lane 11: Mouse testis tissue lysate (40 µg/Lane)
Lane 12: Mouse brain tissue lysate (40 µg/Lane)
Lane 13: Rat testis tissue lysate (40 µg/Lane)
Lane 14: Rat brain tissue lysate (40 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

☑ Knockdown (KD)

Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate (10 µg/Lane)
Lane 2: HAP1-cIAP1 KD cell lysate (10 µg/Lane)

Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 78 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.