Skip to content

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71] - BSA and Azide free

Usd: 649

Specification

Bulk Order Quote | Customization Request

Catalog# HA751100

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71] - BSA and Azide free

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human cIAP1 aa 201-618.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 70 kDa

Positive Control

HT-29 cell lysate, TF-1 cell lysate, HepG2 cell lysate, HeLa cell lysate, THP-1 cell lysate, Jurkat cell lysate, A549 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse testis tissue lysate, Mouse brain tissue lysate, Rat testis tissue lysate, Rat brain tissue lysate.

Conjugation

unconjugated

Clone Number

PSH06-71

Product Features

Form

Liquid

Concentration

1mg/ml

Storage Instructions

Store at 2-8℃. Avoid freeze.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

Target

Function

Baculoviral IAP repeat-containing protein 2 (also known as cIAP1) is a protein that in humans is encoded by the BIRC2 gene. cIAP1 is a member of the Inhibitor of Apoptosis family that inhibit apoptosis by interfering with the activation of caspases.

Background References

1. Zadoroznyj A et al. Cytoplasmic and Nuclear Functions of cIAP1. Biomolecules. 2022 Feb

2. Yang X et al. TRIM56 promotes malignant progression of glioblastoma by stabilizing cIAP1 protein. J Exp Clin Cancer Res. 2022 Dec

Subcellular Location

Cytoplasm, Nucleus.

UNIPROT

SwissProt: Q13490 Human

SwissProt: Q62210 Mouse

Entrez Gene: 60371 Rat

Synonyms

API 1 antibody

API1 antibody

Apoptosis inhibitor 1 antibody

Baculoviral IAP repeat containing 2 antibody

Baculoviral IAP repeat containing protein 2 antibody

Baculoviral IAP repeat-containing protein 2 antibody

BIRC 2 antibody

BIRC2 antibody

BIRC2_HUMAN antibody

C IAP1 antibody

Expand

Images

  • This data was developed using <a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (<a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>) at 1/2,000 dilution.<br /><br />Lane 1: HT-29 cell lysate (20 µg/Lane)<br />Lane 2: TF-1 cell lysate (20 µg/Lane)<br />Lane 3: HepG2 cell lysate (20 µg/Lane)<br />Lane 4: HeLa cell lysate (20 µg/Lane)<br />Lane 5: THP-1 cell lysate (20 µg/Lane)<br />Lane 6: Jurkat cell lysate (20 µg/Lane)<br />Lane 7: A549 cell lysate (20 µg/Lane)<br />Lane 8: HEK-293 cell lysate (20 µg/Lane)<br />Lane 9: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 10: PC-12 cell lysate (20 µg/Lane)<br />Lane 11: Mouse testis tissue lysate (40 µg/Lane)<br />Lane 12: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 13: Rat testis tissue lysate (40 µg/Lane)<br />Lane 14: Rat brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 70 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 2 minutes; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    This data was developed using HA722743, the same antibody clone in a different buffer formulation.
    Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution.

    Lane 1: HT-29 cell lysate (20 µg/Lane)
    Lane 2: TF-1 cell lysate (20 µg/Lane)
    Lane 3: HepG2 cell lysate (20 µg/Lane)
    Lane 4: HeLa cell lysate (20 µg/Lane)
    Lane 5: THP-1 cell lysate (20 µg/Lane)
    Lane 6: Jurkat cell lysate (20 µg/Lane)
    Lane 7: A549 cell lysate (20 µg/Lane)
    Lane 8: HEK-293 cell lysate (20 µg/Lane)
    Lane 9: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 10: PC-12 cell lysate (20 µg/Lane)
    Lane 11: Mouse testis tissue lysate (40 µg/Lane)
    Lane 12: Mouse brain tissue lysate (40 µg/Lane)
    Lane 13: Rat testis tissue lysate (40 µg/Lane)
    Lane 14: Rat brain tissue lysate (40 µg/Lane)

    Predicted band size: 70 kDa
    Observed band size: 70 kDa

    Exposure time: 2 minutes; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />This data was developed using <a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>, the same antibody clone in a different buffer formulation.<br />Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (<a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate (10 µg/Lane)<br />Lane 2: HAP1-cIAP1 KD cell lysate (10 µg/Lane)<br /><br />Predicted band size: 70 kDa<br />Observed band size: 70 kDa<br />Exposure time: 78 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722743" style="font-weight: bold;text-decoration: underline;">HA722743</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    This data was developed using HA722743, the same antibody clone in a different buffer formulation.
    Western blot analysis of cIAP1 on different lysates with Rabbit anti-cIAP1 antibody (HA722743) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate (10 µg/Lane)
    Lane 2: HAP1-cIAP1 KD cell lysate (10 µg/Lane)

    Predicted band size: 70 kDa
    Observed band size: 70 kDa
    Exposure time: 78 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722743) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH02-10]

Application: WB

Reactivity: Human

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71] - BSA and Azide free (Detector)

Application: ELISA(Det)

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH02-10] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-72] - BSA and Azide free (Capture)

Application: ELISA(Cap)

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH02-10] - BSA and Azide free

Application: WB

Reactivity: Human

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-72] - BSA and Azide free

Application: WB,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-72]

Application: WB,IF-Cell,FC

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Biotin Conjugated cIAP1 Recombinant Rabbit Monoclonal Antibody [PSH06-71] - Detector

Application: ELISA(Det),ELISA

Reactivity: Human,Mouse,Rat

Conjugate: Biotin