CASK Mouse Monoclonal Antibody [A1C7] (EM1902-08)
Catalog# EM1902-08
Mouse Monoclonal to CASK
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Knock-Out or Knock-Down validated
Western blot analysis of CASK on different lysates with Mouse anti-CASK antibody (EM1902-08) at 1/2,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD CASK cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD CASK cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 105 kDa
Observed band size: 105 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1902-08) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.