Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07] (ET1608-69)
Catalog# ET1608-69
Rabbit Monoclonal to Caspase-1
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WB
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FC
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IF-Cell
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Human
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Mouse
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Rat
Lane 1: THP-1 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: HL-60 cell lysate
Lane 4: RAW264.7 cell lysate
Lane 5: Mouse spleen lysate
Lane 6: Rat spleen lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1608-69) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Caspase-1 Recombinant Rabbit Monoclonal Antibody [SU40-07] (ET1608-69)
Immunogen
Recombinant protein within human caspase-1 aa 213-404 / 404.
Host
Rabbit
Positive Control
THP-1 cell lysate, HCT 116 cell lysate, HL-60 cell lysate, RAW264.7 cell lysate, Mouse spleen lysate, Rat spleen lysate, U937 cell lysates, PC-12, HL-60, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SU40-07
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:100,000
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IF-Cell
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1:100
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 45 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Caspase-1
SYNONYMS
Caspase1;CASP-1 antibody;CASP1 antibody;CASP1_HUMAN antibody;Caspase1 antibody;Caspase 1 antibody;Caspase-1 subunit p10 antibody;ICE antibody;IL-1 beta-converting enzyme antibody;IL-1BC antibody;IL1 beta converting enzyme antibody;IL1B convertase antibody;Interleukin 1 beta convertase antibody;Interleukin 1B converting enzyme antibody;Interleukin-1 beta convertase antibody;Interleukin-1 beta-converting enzyme antibody;p45 antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase C14A family.
TISSUE SPECIFICITY
Expressed in larger amounts in spleen and lung. Detected in liver, heart, small intestine, colon, thymus, prostate, skeletal muscle, peripheral blood leukocytes, kidney and testis. No expression in the brain.
POST-TRANSLATIONAL MODIFICATION
The two subunits are derived from the precursor sequence by an autocatalytic mechanism.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane.
FUNCTION
Caspase-1, originally designated ICE (for IL-1 converting enzyme), is a member of the group of caspases with large prodomains. Caspase-1 promotes maturation of interleukin IL-1β and interleukin18 (IL-18) by proteolytic cleavage of precursor forms into biologically active pro-inflamatory cytokines. Active caspase-1, a (p20/p10)2 tetramer, is necessary and sufficient for cleavage of precursor IL-1 as well as for induction of apoptosis in some cell lines. The highly conserved family of caspases mediate many of the morphological and biochemical features of apoptosis, including structural dismantling of cell bodies and nuclei, fragmentation of genomic DNA, destruction of regulatory proteins and propagation of other pro-apoptotic molecules. The human Caspase-1 gene maps to chromosome 2q14 and encodes a cytoplasmic protein expressed in liver, heart, skeletal muscle kidney and testis. Caspase-1 has been implicated in inflammation, septic shock, and other situations such as wound healing and the growth of certain leukemias.
CITATIONS
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Lai Yingying,et al
Puerarin enhances TFEB-mediated autophagy and attenuates ROS-induced pyroptosis after ischemic injury of random-pattern skin flaps
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WB
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Human
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Citation
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Liu, Y., Guo, X., Zhang, J., Han, X., Wang, H., Du, F., Zeng, X., & Guo, C.
Protective Effects of the Soluble Receptor for Advanced Glycation End-Products on Pyroptosis during Myocardial Ischemia-Reperfusion
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IHC
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Mouse
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Citation
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Wang Quanzhen,et al
LL-37 improves sepsis-induced acute lung injury by suppressing pyroptosis in alveolar epithelial cells
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WB
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Mouse
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Citation
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Dr. Qi Wang
Hyperglycemia-induced inflamm-aging accelerates gingival senescence via NLRC4 phosphorylation.
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WB
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Mouse
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Citation
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Zhou Peijie,et al
Spike lavender essential oil attenuates hyperuricemia and induced renal injury by modulating the TLR4/NF-κB/NLRP3 signalling pathway
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WB
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Rat
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Citation
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Huiling Yuan,et al
The Molecular Mechanism of Quercetin Improving Cerebral Ischemia Injury by Regulating NLRP3 Inflammasome Mediated Pyroptosis Axis
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WB
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Mouse
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Citation
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Yanan He,et al
Study on the mechanism on Yi-guan-jian decoction alleviating cognitive dysfunction in type 2 diabetes mellitus
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WB
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Rat
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Citation
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Wu, A., Sun, W., & Mou, F.
lncRNA‑MALAT1 promotes high glucose‑induced H9C2 cardiomyocyte pyroptosis by downregulating miR‑141‑3p expression. Molecular medicine reports, 23(4), 259.
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WB
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Rat
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Citation
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Wang Yue,et al
Artemisinin attenuated ischemic stroke induced pyroptosis by inhibiting ROS/TXNIP/NLRP3/Caspase-1 signaling pathway
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WB
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Rat
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Citation
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Discovery of Novel 1,2,3,4-Tetrahydrobenzofuro[2,3-c]pyridine Histone Deacetylase Inhibitors for Efficient Treatment of Hepatocellular Carcinoma
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WB
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Human
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Citation
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Hyperglycemia accelerates inflammaging in the gingival epithelium through inflammasomes activation
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IHC
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WB
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Mouse
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Citation
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Yu Jingyue,et al
Integrating network pharmacology and experimental models to identify notoginsenoside R1 ameliorates atherosclerosis by inhibiting macrophage NLRP3 inflammasome activation
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WB
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Mouse
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Citation
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Renhao Geng,et al
Punicalagin Inhibits African Swine Fever Virus Replication by Targeting Early Viral Stages and Modulating Inflammatory Pathways
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WB
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Sus Scrofa
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Citation
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Wang Jiabin,et al
Comprehensive multi-omics analysis of pyroptosis for optimizing neoadjuvant immunotherapy in patients with gastric cancer
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IHC
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Human
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Citation
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Erythropoietin Attenuates Experimental Contrast-Induced Nephrology: A Role for the Janus Kinase 2/Signal Transducer and Activator of Transcription 3 Signaling Pathway
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WB
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Human
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Citation
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Wang Xiaohong,et al
Deciphering the Therapeutic Potential of SheXiangXinTongNing: Interplay between Gut Microbiota and Brain Metabolomics in a CUMS Mice Model, with a Focus on Tryptophan Metabolism
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WB
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Mouse
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Citation
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Hu Shian,et al
Salvianolic Acid B Alleviates Liver Injury by Regulating Lactate-Mediated Histone Lactylation in Macrophages
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WB
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Mouse
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Citation
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Li, Xin;Wang, Chunchun;Zhu, Jiang;Lin, Qian;Yu, Minjie;Wen, Jiashu;Feng, Jie;Hu, Caihong
Sodium Butyrate Ameliorates Oxidative Stress-Induced Intestinal Epithelium Barrier Injury and Mitochondrial Damage through AMPK-Mitophagy Pathway
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WB
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Pig
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Citation
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Lei, B., Liu, J., Yao, Z., Xiao, Y., Zhang, X., Zhang, Y., & Xu, J.
NF-κB-Induced Upregulation of miR-146a-5p Promoted Hippocampal Neuronal Oxidative Stress and Pyroptosis via TIGAR in a Model of Alzheimer's Disease. Frontiers in cellular neuroscience, 15, 653881.
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WB
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Human
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Citation
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Curcumin attenuates MSU crystal-induced inflammation by inhibiting the degradation of IκBα and blocking mitochondrial damage.
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WB
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Human,Mouse
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Citation
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Liu Li,et al
Ghrelin suppresses hypoxia/reoxygenation-induced H9C2 cell pyroptosis via NLRP3
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WB
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Rat
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Citation
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Wu, Y., Hu, A., Shu, X., Huang, W., Zhang, R., Xu, Y., & Yang, C.
Lactobacillus plantarum postbiotics trigger AMPK-dependent autophagy to suppress Salmonella intracellular infection and NLRP3 inflammasome activation
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WB
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Pig
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Citation
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Aixin Hu , Wenxia Huang , Xin Shu , Shiyue Ma , Caimei Yang , Ruiqiang Zhang , Xiao Xiao , Yanping Wu
Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice
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WB
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Mouse
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Citation
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Sevoflurane preconditioning improves neuroinflammation in cerebral ischemia/reperfusion induced rats through ROS-NLRP3 pathway
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WB
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Rat
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Citation
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Zou Kaiao,et al
Aucubin Alleviates Intervertebral Disc Degeneration by Repressing NF-κB-NLRP3 Inflammasome Activation in Endplate Chondrocytes
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WB
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IHC
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Mouse
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Citation
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Jiang, H., Chen, F., Song, D., Zhou, X., Ren, L., & Zeng, M.
Dynamin-Related Protein 1 Is Involved in Mitochondrial Damage, Defective Mitophagy, and NLRP3 Inflammasome Activation Induced by MSU Crystals
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WB
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Mouse
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Citation
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Wang, Y., Sun, Z., Zhang, H., Song, Y., Wang, Y., Xu, W., & Li, M.
CVB3 Inhibits NLRP3 Inflammasome Activation by Suppressing NF-κB Pathway and ROS Production in LPS-Induced Macrophages
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WB
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Mouse
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Citation
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He, M., Fan, J., Zhou, R., Gao, G., Li, R., Zuo, Y., Li, B., Li, Y., & Sun, T.
NLRP3/Caspase-1-Mediated Pyroptosis of Astrocytes Induced by Antipsychotics Is Inhibited by a Histamine H1 Receptor-Selective Agonist
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WB
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Human
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Citation
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Yi X, Song Y, Xu J, et al
NLRP10 promotes AGEs-induced NLRP1 and NLRP3 inflammasome activation via ROS/MAPK/NF-κB signaling in human periodontal ligament cells
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WB
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Human
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Citation
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Nie, L., Zhao, P., Yue, Z., Zhang, P., Ji, N., Chen, Q., & Wang, Q.
Diabetes induces macrophage dysfunction through cytoplasmic dsDNA/AIM2 associated pyroptosis. Journal of leukocyte biology, 10.1002/JLB.3MA0321-745R. Advance online publication.
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WB
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Mouse
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Citation
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Xin, Y., Wang, W., Mao, E., Yang, H., & Li, S.
Targeting NLRP3 Inflammasome Alleviates Synovitis by Reducing Pyroptosis in Rats with Experimental Temporomandibular Joint Osteoarthritis
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IHC
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WB
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Rat
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Citation
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Neuroprotective effects of Jie-du-huo-xue decoction on microglia pyroptosis after cerebral ischemia and reperfusion——From the perspective of glial-vascular unit
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WB
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Rat
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Citation
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Tan, J., Dong, L., Jiang, Z., Tan, L., Luo, X., Pei, G., Qin, A., Zhong, Z., Liu, X., Tang, Y., & Qin, W.
Probiotics ameliorate IgA nephropathy by improving gut dysbiosis and blunting NLRP3 signaling
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WB
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Mouse
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Citation
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Lai Yingying,et al
Dihydrocapsaicin suppresses the STING-mediated accumulation of ROS and NLRP3 inflammasome and alleviates apoptosis after ischemia-reperfusion injury of perforator skin flap
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WB
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Rat
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Citation
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Ling Zhao,et al
The AGEs/RAGE Signaling Pathway Regulates NLRP3-Mediated Neuronal Pyroptosis After MCAO Injury in Lepr−/− Obese Rats
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WB
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Rat
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Citation
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Zhang Zizi,et al
The hijacking of HBV by small extracellular vesicles inhibits M1 macrophages to facilitate immune evasion
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WB
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Human
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Citation
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