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CD34 Recombinant Rabbit Monoclonal Antibody

Usd: 209

Specification

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Catalog# IRS050

CD34 Recombinant Rabbit Monoclonal Antibody

Application

  • mIHC

Reactivity

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

CD34 Recombinant Rabbit Monoclonal Antibody

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD34 aa 336-385 / 385.

Species Reactivity

Mouse

Validated Applications

mIHC

Molecular Weight

Predicted band size: 41 kDa

Positive Control

Mouse brain tissue, mouse liver tissue, mouse spleen tissue.

Conjugation

unconjugated

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • mIHC

  • 1:100

Target

Function

CD34 is a heavily glycosylated, transmembrane glycoprotein that is expressed on the surface of lymphohematopoietic stem and progenitor cells, small-vessel endothelial cells, embryonic fibroblasts and some cells in fetal and adult nervous tissue. CD34 antigen expression is highest in the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. The CD34 cytoplasmic domain has an intracellular domain that contains consensus sites for activated protein kinase C (PKC) phosphorylation as well as serine, threonine and tyrosine phosphorylation consensus sites.

Background References

1. Lin, SZ. et al. 2015. Emodin inhibits angiogenesis in pancreatic cancer by regulating the transforming growth factor-β/drosophila mothers against decapentaplegic pathway and angiogenesis-associated microRNAs. Molecular medicine reports. 12: 5865-71.

2. Corradi, LS. et al. 2013. Structural and ultrastructural evidence for telocytes in prostate stroma. J. Cell. Mol. Med. 17: 398-406.

Sequence Similarity

Belongs to the CD34 family.

Tissue Specificity

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational Modification

Highly glycosylated.; Phosphorylated on serine residues by PKC.

Subcellular Location

Membrane.

UNIPROT

SwissProt: Q64314 Mouse

Synonyms

CD34 antibody

CD34 antigen antibody

CD34 molecule antibody

CD34_HUMAN antibody

Cluster designation 34 antibody

Hematopoietic progenitor cell antigen CD34 antibody

HPCA1 antibody

Mucosialin antibody

OTTHUMP00000034733 antibody

OTTHUMP00000034734 antibody

Images

  • mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with SOX2, TBR1 (<a href="/products/IRS070" style="font-weight: bold;text-decoration: underline;">IRS070</a>), Olig2 (<a href="/products/IRS067" style="font-weight: bold;text-decoration: underline;">IRS067</a>), CD34 (<a href="/products/IRS050" style="font-weight: bold;text-decoration: underline;">IRS050</a>) and GFAP (<a href="/products/IRS069" style="font-weight: bold;text-decoration: underline;">IRS069</a>) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900802" style="font-weight: bold;text-decoration: underline;">900802</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with SOX2, TBR1 (IRS070), Olig2 (IRS067), CD34 (IRS050) and GFAP (IRS069) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (<a href="/products/IRS050" style="font-weight: bold;text-decoration: underline;">IRS050</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse brain tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (<a href="/products/IRS050" style="font-weight: bold;text-decoration: underline;">IRS050</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

  • mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (<a href="/products/IRS050" style="font-weight: bold;text-decoration: underline;">IRS050</a>) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (<a href="/products/900808" style="font-weight: bold;text-decoration: underline;">900808</a>). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

    mIHC analysis of mouse spleen tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD34 antibody (IRS050) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"