Claudin 5 Recombinant Rabbit Monoclonal Antibody [JM11-22] (ET1703-58)
Catalog# ET1703-58
Rabbit Monoclonal to Claudin 5
-
WB
-
IHC-P
-
Human
Lane 1: Human brain tissue lysate
Lane 2: Human lung tissue lysate
Lane 3: Human kidney tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 20 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-58) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Claudin 5 Recombinant Rabbit Monoclonal Antibody [JM11-22] (ET1703-58)
Immunogen
Synthetic peptide within human claudin 5 aa 191-214 / 218.
Host
Rabbit
Positive Control
Human brain tissue lysate, human lung tissue lysate, human kidney tissue lysate, human tonsil tissue, human kidney tissue, human lung tissue, human uterus tissue, human lymph nodes tissue
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-22
RRID
APPLICATION DILUTION
-
WB
-
1:5,000
-
IHC-P
-
1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Claudin 5
SYNONYMS
Androgen withdrawal and apoptosis induced protein RVP1 like antibody;AWAL antibody;BEC 1 antibody;BEC1 antibody;Claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) antibody;Claudin-5 antibody;Claudin5 antibody;CLD5_HUMAN antibody;CLDN 5 antibody;Cldn5 antibody;CPETR L1 antibody;CPETRL 1 antibody;CPETRL1 antibody;TMDVCF antibody;TMVCF antibody;Transmembrane protein deleted in VCFS antibody;Transmembrane protein deleted in velocardiofacial syndrome antibody
SEQUENCE SIMILARITIES
Belongs to the claudin family.
SUBCELLULAR LOCATION
Cell junction. Cell membrane.
FUNCTION
The claudin superfamily consists of many structurally related proteins in humans. These proteins are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecule. Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Claudin expression is highly restricted to specfic regions of different tissues and may have an important role in transcellular transport through tight junctions. Claudin-5 is expressed in the endothelial junctions of the rat liver and in junctions of acinar cells of the pancreas. Human Claudin-5 is abundantly expressed in adult lung, heart and skeletal muscle and is deleted in patients with velocardiofacial syndrone, which is characterized by cleft palate, facial dysmorphology and conotruncal heart defects.
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