Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05] (ET1609-62)
Catalog# ET1609-62
Rabbit Monoclonal to Cytokeratin 7
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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FC
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mIHC
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Human
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Mouse
Lane 1/2: Wild-type Hela whole cell lysate (20 µg).
Lane 3/4: Cytokeratin 7 fragment 1 knockdown Hela whole cell lysate (20 µg).
Lane 5/6: Cytokeratin 7 fragment 2 knockdown Hela whole cell lysate (20 µg).
ET1609-62 was shown to specifically react with Cytokeratin 7 in wild-type Hela cells. Weakened bands were observed when Cytokeratin 7 knockdown samples were tested. Wild-type and Cytokeratin 7 knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1609-62, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH , ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cytokeratin 7 Recombinant Rabbit Monoclonal Antibody [ST50-05] (ET1609-62)
Immunogen
Synthetic peptide within human cytokeratin 7 aa 18-67.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, A549, human breast tissue, human breast carcinoma tissue, SK-Br-3, human liver tissue, human gastric cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
ST50-05
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:100
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IF-Tissue
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1:400
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IHC-P
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1:1,000-1:2,000
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FC
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1:1,000
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IP
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Use at an assay dependent concentration.
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mIHC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 51 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 7
SYNONYMS
CK 7 antibody;CK-7 antibody;CK7 antibody;Cytokeratin 7 antibody;Cytokeratin-7 antibody;D15Wsu77e antibody;K2C7 antibody;K2C7_HUMAN antibody;K7 antibody;Keratin 7 antibody;Keratin 7, type II antibody;Keratin type II cytoskeletal 7 antibody;Keratin, 55K type II cytoskeletal antibody;Keratin, simple epithelial antibody;Keratin, simple epithelial type I, K7 antibody;Keratin, type II cytoskeletal 7 antibody;Keratin-7 antibody;Krt2-7 antibody;KRT7 antibody;MGC11625 antibody;MGC129731 antibody;MGC3625 antibody;Sarcolectin antibody;SCL antibody;Type II mesothelial keratin K7 antibody;Type-II keratin Kb7 antibody;Cytokeratin7
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Expressed in cultured epidermal, bronchial and mesothelial cells but absent in colon, ectocervix and liver. Observed throughout the glandular cells in the junction between stomach and esophagus but is absent in the esophagus.
POST-TRANSLATIONAL MODIFICATION
Arg-20 is dimethylated, probably to asymmetric dimethylarginine.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins are useful markers of tissue differentiation and, in addition, they aid in the characterization of malignant tumors. Cytokeratin 7 (also known as sarcolectin) agglutinates normal and transformed cells with a high affinity for simple sugars. Cytokeratin 7 also inhibits the synthesis of interferon-dependent secondary proteins thus reversing the antiviral effect of interferon induction and restoring cells to their status ad primum. In normal and transformed cells, Cytokeratin 7 localizes to the membrane.
CITATIONS
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Shi Yaxin,et al
Paris polyphylla ethanol extract and polyphyllin I ameliorate adenomyosis by inhibiting epithelial–mesenchymal transition
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IF
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Mouse
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Citation
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Zhu, J., Song, G., Zhou, X., Han, T. L., Yu, X., Chen, H., Mansell, T., Novakovic, B., Baker, P. N., Cannon, R. D., Saffery, R., Chen, C., & Zhang, H.
CD39/CD73 Dysregulation of Adenosine Metabolism Increases Decidual Natural Killer Cell Cytotoxicity: Implications in Unexplained Recurrent Spontaneous Abortion
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IHC
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Human
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Citation
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Wenming Xu
Deficiency of DICER reduces the invasion ability of trophoblasts and impairs the pro-angiogenic effect of trophoblast-derived microvesicles
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IF
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IHC
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human
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Citation
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