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DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31] (ET1703-05)

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Catalog# ET1703-05

Rabbit Monoclonal to DDIT3

Tested for

  • WB

  • IHC-P

  • FC

Reacts with

  • Human

  • Mouse

  • Rat

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
This product has been cited in peer reviewed publications, see list HERE

Specifications

Product Type

Recombinant Rabbit monoclonal primary

Product Name

DDIT3 Recombinant Rabbit Monoclonal Antibody [JM10-31] (ET1703-05)

Immunogen

Synthetic peptide within human ddit3 aa 135-169 / 169.

Host

Rabbit

Positive Control

SW480 cell lysate, HeLa cell lysate, LOVO cell lysate, PC-12 cell lysate, human breast carcinoma tissue, human stomach carcinoma tissue, human pancreas tissue, mouse pancreas tissue, mouse brain tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM10-31

RRID

AB_3070363

APPLICATION DILUTION

  • WB

  • 1:5,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 19 kDa

Isotype

IgG

TARGET

UNIPROT #

P35638

PROTEIN NAME

DDIT3

SYNONYMS

C/EBP homologous protein antibody;C/EBP Homology Protein antibody;C/EBP zeta antibody;C/EBP-homologous protein 10 antibody;C/EBP-homologous protein antibody;CCAAT/enhancer binding protein homologous protein antibody;CEBPZ antibody;CHOP 10 antibody;CHOP antibody;CHOP-10 antibody;CHOP10 antibody;DDIT 3 antibody;DDIT-3 antibody;Ddit3 antibody;DDIT3_HUMAN antibody;DNA Damage Inducible Transcript 3 antibody;DNA damage-inducible transcript 3 protein antibody;GADD 153 antibody;GADD153 antibody;Growth Arrest and DNA Damage Inducible Protein 153 antibody;Growth arrest and DNA damage inducible protein GADD153 antibody;Growth arrest and DNA damage-inducible protein GADD153 antibody;MGC4154 antibody

SEQUENCE SIMILARITIES

Belongs to the bZIP family.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated, leading to its degradation by the proteasome.; Phosphorylation at serine residues by MAPK14 enhances its transcriptional activation activity while phosphorylation at serine residues by CK2 inhibits its transcriptional activation activity.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

DNA damage-inducible transcript 3, also known as C/EBP homologous protein (CHOP), is a pro-apoptotic transcription factor that is encoded by the DDIT3 gene. It is a member of the CCAAT/enhancer-binding protein (C/EBP) family of DNA-binding transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis and has an important role in the cell's stress response. The regulation of CHOP expression plays an important role in metabolic diseases and in some cancers through its function in mediating apoptosis. The regulation of CHOP expression could be a potential approach to affecting cancer cells through the induction of apoptosis. In the intestinal epithelium, CHOP has been demonstrated to be downregulated under inflammatory conditions (in inflammatory bowel diseases and experimental models of colitis). In this context, CHOP seems to rather regulate the cell cycle than apoptotic processes. Mutations or fusions of CHOP (e.g. with FUS to form FUS-CHOP) can cause Myxoid liposarcoma.

CITATIONS

  • Li Xuecong,et al

    Endoplasmic reticulum stress is attenuated by glycolysis in lymphatic malformations

  • Cui, Y. J., Chen, L. Y., Zhou, X., Tang, Z. N., Wang, C., & Wang, H. F.

    Heat stress induced IPEC-J2 cells barrier dysfunction through endoplasmic reticulum stress mediated apoptosis by p-eif2α/CHOP pathway

  • Lingman Wang,et al

    Particulate matter induced cognitive impairments via endoplasmic reticulum stress-mediated damage to mitochondria-associated endoplasmic reticulum membranes in immature rats

  • Tunicamycin induces depression-like behaviors in male rats, accompanied by initiated chaperon-mediated autophagy and decreased synaptic protein expression in the hippocampus

  • Prof. Xingxu Huang,Prof. Jiajie Hou,Prof. Qiang Xia

    Intracellular XBP1-IL-24 axis dismantles cytotoxic unfolded protein response in the liver

  • Feng Juan,et al

    Discovery of novel peptide-dehydroepiandrosterone hybrids inducing endoplasmic reticulum stress with effective in vitro and in vivo anti-melanoma activities

  • GRAMD4 regulates PEDV-induced cell apoptosis inhibiting virus replication via the endoplasmic reticulum stress pathway

  • Deng H, Chen W, Zhang B, et al

    Excessive ER-phagy contributes to ochratoxin A-induced apoptosis

  • Zheng, Z., Zhang, X., Huang, B., Liu, J., Wei, X., Shan, Z., Wu, H., Feng, Z., Chen, Y., Fan, S., Zhao, F., & Chen, J.

    Site-1 protease controls osteoclastogenesis by mediating LC3 transcription. Cell death and differentiation, 28(6), 2001–2018.

  • Porcine circovirus type 2 induces CHOP-ERO1α-ROS-mediated apoptosis in PK-15 cells

  • Porcine epidemic diarrhea virus activates PERK-ROS axis to benefit its replication in Vero E6 cells

  • Jinliang Nan,Yinuo Lin

    Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. Journal of cellular physiology, 234(11), 21166–21181.

    • WB

    • IF

    • Human coronary artery endothelial cells (HCAECs)

    • Citation

  • Gankun Yuan,et al

    Novel immunogenic cell death inducer combined with autophagy inhibitor to amplify photodynamic synergistic immunotherapy for triple-negative breast cancer

  • Zheng Zeyu,et al

    In vivo therapy of osteosarcoma using anion transporters-based supramolecular drugs

  • Nrf2 loss of function exacerbates endoplasmic reticulum stress-induced apoptosis in TBI mice

  • Cao, X., Shu, Y., Chen, Y., Xu, Q., Guo, G., Wu, Z., Shao, M., Zhou, Y., Chen, M., Gong, Y., Li, C., Shi, Y., & Bu, H.

    Mettl14-Mediated m6A Modification Facilitates Liver Regeneration by Maintaining Endoplasmic Reticulum Homeostasis. Cellular and molecular gastroenterology and hepatology, S2352-345X(21)00072-2. Advance online publication.

  • Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice

  • Jin Tingting,et al

    HDAC1 Promotes Mitochondrial Pathway Apoptosis and Inhibits the Endoplasmic Reticulum Stress Response in High Glucose-Treated Schwann Cells via Decreased U4 Spliceosomal RNA

  • Corn peptides ameliorate nonalcoholic fatty liver disease by suppressing endoplasmic reticulum stress via the AMPKα/Sirt1 pathway in vivo and in vitro

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