DYKDDDDK Tag ( FLAG ) Rabbit Polyclonal Antibody (0912-1)
Catalog# 0912-1
Rabbit Polyclonal to DYKDDDDK Tag ( FLAG )
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WB
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IP
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Species independent
Specifications
Product Type
Rabbit polyclonal primary
Product Name
DYKDDDDK Tag ( FLAG ) Rabbit Polyclonal Antibody (0912-1)
Immunogen
Synthetic peptide immune sequence is cdykddddk.
Host
Rabbit
Positive Control
DYKDDDDK Tag recombinant protein, N-terminal FLAG-tagged recombinant protein, C-terminal FLAG-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IP
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1:10-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
DYKDDDDK Tag ( FLAG )
SYNONYMS
DDDDK epitope tag antibody;DDDK antibody;ddk antibody;DYKDDDDK antibody;DYKDDDDK epitope tag antibody;DYKDDDDK tag antibody;ECS epitope tag antibody;ECS tag antibody;Enterokinase Cleavage Site epitope tag antibody;Enterokinase Cleavage Site tag antibody;FLAG antibody;FLAG tag antibody
FUNCTION
The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. Due to its small size, it is unlikely to affect the tagged protein's biochemical properties. This antibody recognizes the DYKDDDDK peptide fused to either the amino- or carboxy-terminus of targeted proteins.
CITATIONS
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Wu, X., Lai, Y., Rao, S., Lv, L., Ji, M., Han, K., Weng, J., Lu, Y., Peng, J., Lin, L., Wu, G., Chen, J., Yan, F., & Zheng, H.
Genome-Wide Identification Reveals That Nicotiana benthamiana Hypersensitive Response (HR)-Like Lesion Inducing Protein 4 (NbHRLI4) Mediates Cell Death and Salicylic Acid-Dependent Defense Responses to Turnip Mosaic Virus. Frontiers in plant science, 12, 627315.
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WB
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Nicotiana tabacum
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Luan Yi,et al
Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection
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WB
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Human
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Wang, J., Ding, Y., Li, D., Zhu, N., Nishiyama, A., & Yuan, Y.
(Pro)renin receptor promotes colorectal cancer progression through inhibiting the NEDD4L-mediated Wnt3 ubiquitination and modulating gut microbiota
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WB
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Human
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Wei Liu
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction
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WB
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HEK293 cells
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Zhou Zhao,et al
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ChIP
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Human
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Di Wang
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WB
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IP
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Jian Chen;Fengdong Zhao
The RCAN1.4-calcineurin/NFAT signaling pathway is essential for hypoxic adaption of intervertebral discs
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WB
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IP
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human
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Zhou H, Yan Y, Gao J, et al
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MSX3 switches microglia polarization and protects from inflammation-induced demyelination
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ChIP
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Mouse
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Kaifu T
Dicer interacts with SIRT7 and regulates H3K18 deacetylation in response to DNA damaging agents
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WB
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human
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Deng, T., Hu, B., Wang, X., Yan, Y., Zhou, J., Lin, L., Xu, Y., Zheng, X., & Zhou, J.
DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication
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WB
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Human
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Yu Miaomiao,et al
Hsa_circ_0000825 promotes the progression of laryngeal squamous cell carcinoma by sponging miR-766 and interacting with ELAVL1
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RIP
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Zhou, J., Wang, Y., Zhou, L., Qiu, Y., Zhao, J., Dai, B., Feng, X., Hou, L., & Liu, J.
Interaction Network of Porcine Circovirus Type 3 and 4 Capsids with Host Proteins
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WB
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Human
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Citation
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Jinyan Gu; Jiyong Zhou
Nucleolar protein NPM1 is essential for circovirus replication by binding to viral capsid
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WB
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CoIP
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human
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Citation
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Jiyong Zhou
Protein Interactions Network of Porcine Circovirus Type 2 Capsid With Host Proteins
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WB
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pig
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Citation
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Zhou, J., Qiu, Y., Zhu, N., Zhou, L., Dai, B., Feng, X., Hou, L., & Liu, J.
The Nucleolar Localization Signal of Porcine Circovirus Type 4 Capsid Protein Is Essential for Interaction With Serine-48 Residue of Nucleolar Phosphoprotein Nucleophosmin-1
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IP
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Citation
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Qin Liu
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and StressActivated Protein Kinase 1-Dependent Manner
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WB
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Escherichia coli
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Citation
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Zhou, J., Li, J., Li, H., Zhang, Y., Dong, W., Jin, Y., Yan, Y., Gu, J., & Zhou, J.
The serine-48 residue of nucleolar phosphoprotein nucleophosmin-1 plays critical role in subcellular localization and interaction with porcine circovirus type 3 capsid protein. Veterinary research, 52(1), 4.
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WB
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Citation
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王迪
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages
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WB
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None
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Citation
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Wei Liu;Chun Tang
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress
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IP
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CO-IP
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human
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Citation
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Deng T, Du L, Ding S, et al
Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein
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WB
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Human
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Citation
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Transforming acidic coiled-coil containing protein 3 suppresses influenza A virus replication by impeding viral endosomal trafficking and nuclear import
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IF-cell
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Citation
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Guoqiang Xu;Xiangao Huang
Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function
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IP
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human
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Citation
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Deng, T., Hu, B., Wang, X., Ding, S., Lin, L., Yan, Y., Peng, X., Zheng, X., Liao, M., Jin, Y., Dong, W., Gu, J., & Zhou, J.
TRAF6 autophagic degradation by avibirnavirus VP3 inhibits antiviral innate immunity via blocking NFKB/NF-κB activation
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WB
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Chicken
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Citation
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Zhao, S., Chen, G., Kong, X., Chen, N., & Wu, X.
BmNPV p35 Reduces the Accumulation of Virus-Derived siRNAs and Hinders the Function of siRNAs to Facilitate Viral Infection
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Liu Shengde,et al
Claudin-18.2 mediated interaction of gastric Cancer cells and Cancer-associated fibroblasts drives tumor progression
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WB
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Human
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Mouse
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Citation
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Label‐free proteomic dissection on dptP‐deletion mutant uncovers dptP involvement in strain growth and daptomycin tolerance of Streptomyces roseosporus
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WB
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Citation
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Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C.
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
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WB
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Citation
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Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB
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WB
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Human
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Citation
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Dai Shuying,et al
Taxus chinensis var. mairei (Lemée et Lévl) Cheng et L.K. Fu overcomes the resistance to osimertinib in EGFR-mutant non-small-cell lung cancer via suppression of ERK1/2-related cholesterol biosynthesis
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WB
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Citation
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Lei Hou,et al
RSAD2 suppresses viral replication by interacting with the Senecavirus A 2 C protein
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WB
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Human
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Citation
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jwzhou@njmu.edu.cn
JWA inhibits nicotine-induced lung cancer stemness and progression through CHRNA5/AKT-mediated JWA/SP1/CD44 axis
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WB
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Mouse
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Citation
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Jihong Sun6 & Xiaojian Wang1
Downregulated NDR1 protein kinase inhibits innate immune response by initiating an miR146a-STAT1 feedback loop
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WB
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mouse
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Citation
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Wu, Y., Zhang, C., Liu, X., He, Z., Shan, B., Zeng, Q., Zhao, Q., Zhu, H., Liao, H., Cen, X., Xu, X., Zhang, M., Hou, T., Wang, Z., Yan, H., Yang, S., Sun, Y., Chen, Y., Wu, R., Xie, T., … Xia, H.
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
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WB
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Human
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Citation
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Zhou, J., Wang, Y., Qiu, Y., Wang, Y., Yang, X., Liu, C., Shi, Y., Feng, X., Hou, L., & Liu, J.
Contribution of DEAD-Box RNA Helicase 21 to the Nucleolar Localization of Porcine Circovirus Type 4 Capsid Protein
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WB
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Human
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Citation
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Mu Mingshan,et al
FAM188B promotes the growth, metastasis, and invasion of hepatocellular carcinoma by targeting the HRNPA1/PKM2 axis
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WB
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Human
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Citation
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Jin Kai,et al
BZW2 promotes malignant progression in lung adenocarcinoma through enhancing the ubiquitination and degradation of GSK3β
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WB
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IP
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Human
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Citation
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Ma, L., Li, Y., Luo, R., Wang, Y., Cao, J., Fu, W., Qian, B., Zheng, L., Tang, L., Lv, X., Zheng, L., Liang, G., & Chen, L.
Discovery of a Selective and Orally Bioavailable FGFR2 Degrader for Treating Gastric Cancer
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WB
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Mouse
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Human
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ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade
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WB
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Human
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Citation
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Baojian Wu
E4bp4 Regulates Carboxylesterase 2 Enzymes through Repression of the Nuclear Receptor Rev-erbα in Mice
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WB
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Mouse
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Citation
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Jihui Ping
Features of Nuclear Export Signals of NS2 Protein of Influenza D Virus
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COIP
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human
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Citation
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Deng, T., Hu, B., Wang, X., Lin, L., Zhou, J., Xu, Y., Yan, Y., Zheng, X., & Zhou, J.
Inhibition of Antiviral Innate Immunity by Avibirnavirus VP3 via Blocking TBK1-TRAF3 Complex Formation and IRF3 Activation. mSystems, 6(3), e00016-21.
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WB
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Human
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Citation
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Acetyl-coenzyme A acetyltransferase 1 promotes brown adipogenesis by activating the AMPK-PGC1α signaling pathway
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WB
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Mouse
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Citation
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Zhao, L., Liu, Q., Huang, J., Lu, Y., Zhao, Y., & Ping, J.
TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus
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WB
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Human
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Citation
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Zhou, J., Qiu, Y., Zhao, J., Wang, Y., Zhu, N., Wang, D., Cui, Y., Guo, J., Sun, T., Ji, Y., Wu, Z., Zeng, P., Li, J., Feng, X., Hou, L., & Liu, J.
The Network of Interactions between the Porcine Epidemic Diarrhea Virus Nucleocapsid and Host Cellular Proteins
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WB
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Pig
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Citation
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