Dynamin 1 Mouse Monoclonal Antibody [A1B2] (EM1901-42)
Catalog# EM1901-42
Mouse Monoclonal to Dynamin 1
Tested for
-
WB
-
IHC-P
-
IF-Cell
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of Dynamin 1 on different lysates with Mouse anti-Dynamin 1 antibody (EM1901-42) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 11 seconds; Lane 3: 49 seconds; Lane 4-6: 2 minutes 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: SH-SY5Y cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (40 µg/Lane)
Lane 5: Rat brain tissue lysate (40 µg/Lane)
Lane 6: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 97 kDa
Observed band size: 100 kDa
Exposure time: Lane 1-2: 11 seconds; Lane 3: 49 seconds; Lane 4-6: 2 minutes 43 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-42) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
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