ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53] (ET1705-56)
Catalog# ET1705-56
Rabbit Monoclonal to ENO1
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WB
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FC
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IF-Tissue
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (20 µg/Lane)
Lane 6: Rat brain tissue lysate (20 µg/Lane)
Lane 7: Zebrafish tissue lysate (20 µg/Lane)
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ENO1 Recombinant Rabbit Monoclonal Antibody [JM63-53] (ET1705-56)
Immunogen
Synthetic peptide within human eno1 aa 1-50 / 434.
Host
Rabbit
Positive Control
HeLa cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, Zebrafish tissue lysate, human lung cancer tissue, HeLa, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM63-53
RRID
APPLICATION DILUTION
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WB
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1:2,000
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FC
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1:1,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:1,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ENO1
SYNONYMS
2 phospho D glycerate hydro lyase antibody;2-phospho-D-glycerate hydro-lyase antibody;Alpha enolase antibody;Alpha enolase like 1 antibody;Alpha-enolase antibody;C myc promoter binding protein antibody;C-myc promoter-binding protein antibody;EC 4.2.1.11 antibody;eno1 antibody;ENO1L1 antibody;ENOA_HUMAN antibody;Enolase 1 (alpha) antibody;Enolase 1 (alpha) like 1 antibody;Enolase 1 antibody;Enolase alpha antibody;MBP 1 antibody;MBP-1 antibody;MBP1 antibody;MBPB1 antibody;MPB 1 antibody;MPB-1 antibody;MPB1 antibody;MYC promoter binding protein 1 antibody;NNE antibody;Non neural enolase antibody;Non-neural enolase antibody;Phosphopyruvate hydratase antibody;Plasminogen binding protein antibody;Plasminogen-binding protein antibody;PPH antibody;Tau crystallin antibody
SEQUENCE SIMILARITIES
Belongs to the enolase family.
TISSUE SPECIFICITY
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
DEVELOPMENTAL STAGE
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
POST-TRANSLATIONAL MODIFICATION
ISGylated.; Lysine 2-hydroxyisobutyrylation (Khib) by p300/EP300 activates the phosphopyruvate hydratase activity.
SUBCELLULAR LOCATION
Cell membrane, Cytoplasm, Membrane, Nucleus.
FUNCTION
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
CITATIONS
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He, Y., Wang, X., Lu, W., Zhang, D., Huang, L., Luo, Y., Xiong, L., Li, H., Zhang, P., Li, Q., & Liang, S.
PGK1 contributes to tumorigenesis and sorafenib resistance of renal clear cell carcinoma via activating CXCR4/ERK signaling pathway and accelerating glycolysis
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WB
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Human
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Citation
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Tang, Y., Jin, Y. H., Li, H. L., Xin, H., Chen, J. D., Li, X. Y., & Pan, Y. F.
PBRM1 deficiency oncogenic addiction is associated with activated AKT–mTOR signalling and aerobic glycolysis in clear cell renal cell carcinoma cells
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WB
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Human
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Citation
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