iFluor™ 594 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
Catalog# HA720119F
iFluor™ 594 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
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IF-Tissue
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Human
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Rat
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.
Species Reactivity
Human, Rat
Validated Applications
IF-Tissue
Molecular Weight
Predicted band size: 59 kDa
Positive Control
Human skin tissue, rat skin tissue.
Conjugation
iFluor™ 594
Clone Number
ST05-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:100-1:400
Target
Function
Keratin, type I cytoskeletal 10 also known as cytokeratin-10 (CK-10) or keratin-10 (K10) is a protein that in humans is encoded by the KRT10 gene.]Keratin 10 is a type I keratin.Keratin-10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21.
Background References
1. Tabuchi Y et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim 63:31-44 (2014).
2. Zhu XJ et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687 (2014).
Subcellular Location
Cytoplasm, secreted.
Synonyms
BCIE antibody
BIE antibody
CK 10 antibody
CK10 antibody
CK-10 antibody
Cytokeratin-10 antibody
EHK antibody
K10 antibody
K1C10_HUMAN antibody
Keratin 10 antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 10 (HA720119F, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720119F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Cytokeratin 10 (HA720119F, red) at 1/200 dilution at +4℃ overnight, washed with PBS.
Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720119F, red) at 1/100 dilution and Vimentin (EM0401, green) at 1/400 dilution at +4℃ overnight, washed with PBS.
Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue). -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720119F), Cytokeratin 5 (HA720129F) and Cytokeratin 1 (HA720141F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution, Cytokeratin 5 (HA720129F, green) at 1/100 dilution and Cytokeratin 1 (HA720141F, red) at 1/400 dilution overnight at 4 ℃, washed with PBS.
DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"