Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09] (ET1606-49)
Catalog# ET1606-49
Rabbit Monoclonal to Integrin beta 3
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
Lysates/proteins at 15 µg/Lane.
Predicted band size: 87 kDa
Observed band size: 100 kDa
Exposure time: 1 minute 40 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1606-49) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Integrin beta 3 Recombinant Rabbit Monoclonal Antibody [SJ19-09] (ET1606-49)
Immunogen
Synthetic peptide within human integrin beta 3 aa 729-788 / 788.
Host
Rabbit
Positive Control
U-87 MG cell lysates, HepG2, human spleen tissue, mouse testis tissue, human liver carcinoma tissue, human breast carcinoma tissue, mouse colon tissue, U87-MG-si-NT cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SJ19-09
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:400-1:1,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 87 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Integrin beta 3
SYNONYMS
BDPLT16 antibody;BDPLT2 antibody;CD 61 antibody;CD61 antibody;CD61 antigen antibody;GP3A antibody;GPIIIa antibody;GT antibody;HPA 1 antibody;HPA 4 antibody;Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody;Integrin beta chain beta 3 antibody;Integrin beta-3 antibody;ITB3_HUMAN antibody;ITG B3 antibody;ITGB 3 antibody;ITGB3 antibody;NAIT antibody;Platelet fibrinogen receptor beta subunit antibody;Platelet fibrinogen receptor, beta subunit antibody;Platelet glycoprotein IIIa antibody;Platelet glycoprotein IIIa precursor antibody;Platelet membrane glycoprotein IIIa antibody;PTP antibody
SEQUENCE SIMILARITIES
Belongs to the integrin beta chain family.
TISSUE SPECIFICITY
Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
SUBCELLULAR LOCATION
Cell membrane, Cell projection, Cell junction.
FUNCTION
Integrins are heterodimers composed of noncovalently associated transmembrane a and b subunits. The 16 a and 8 b subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors; both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.
CITATIONS
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Nagpal, A., Needham, K., Lane, D. J. R., Ayton, S., Redvers, R. P., John, M., Selistre-de-Araujo, H. S., Denoyer, D., & Pouliot, N.
Integrin αvβ3 Is a Master Regulator of Resistance to TKI-Induced Ferroptosis in HER2-Positive Breast Cancer
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WB
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Human
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Citation
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Ma, Y., Shi, X., Zhao, H., Song, R., Zou, H., Zhu, J., & Liu, Z.
Potential mechanisms of osteoprotegerin-induced damage to osteoclast adhesion structures via P2X7R-mediated MAPK signaling
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WB
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Mouse
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Citation
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Rongrong Li,et al
Phosphatidylserine-mediated uptake of extracellular vesicles by hepatocytes ameliorates liver ischemia-reperfusion injury
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WB
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Mouse
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Citation
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Xiangqin Tian,et al
Knockdown of ANO1 decreases TGF-β- and IL-6-induced adhesion and migration of cardiac fibroblasts by inhibiting the expression of integrin and focal adhesion kinase
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WB
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Rat
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Citation
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