mSin3A Rabbit Polyclonal Antibody
Catalog# ER2001-38
mSin3A Rabbit Polyclonal Antibody
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WB
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Human
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Mouse
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Rat
Predicted species support after-sales service
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unconjugated
Overview
Product Name
mSin3A Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within human mSin3A aa 1-50.
Species Reactivity
Human (Predicted: Mouse, Rat)
Predicted species support after-sales service
Validated Applications
WB
Molecular Weight
145 kDa
Positive Control
HL-60 cell lysate, A549 cell lysate, Siha cell lysate.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:500-1:1,000
Target
Function
Acts as a transcriptional repressor. Corepressor for REST. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Also interacts with MXD1-MAX heterodimers to repress transcription by tethering SIN3A to DNA. Acts cooperatively with OGT to repress transcription in parallel with histone deacetylation. Involved in the control of the circadian rhythms. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation. Cooperates with FOXK1 to regulate cell cycle progression probably by repressing cell cycle inhibitor genes expression (By similarity). Required for cortical neuron differentiation and callosal axon elongation (By similarity).
Background References
1. Yang X. et. al. Recruitment of O-GlcNAc transferase to promoters by corepressor mSin3A: coupling protein O-GlcNAcylation to transcriptional repression. Cell 110:69-80(2002).
Subcellular Location
Nucleus.
UNIPROT
Synonyms
AW553200 antibody
DKFZP434K2235 antibody
FLJ90319 antibody
Histone deacetylase complex subunit Sin 3a antibody
Histone deacetylase complex subunit Sin3a antibody
KIAA0700 antibody
Kiaa4126 antibody
mKIAA4126 antibody
Paired amphipathic helix protein Sin 3a antibody
Paired amphipathic helix protein Sin3a antibody
ExpandImages
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Western blot analysis of mSin3A on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HL-60 cell lysate
Lane 2: A549 cell lysate
Lane 3: Siha cell lysate
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
mSin3A Recombinant Rabbit Monoclonal Antibody [JA94-31]
Application: WB,IF-Cell,IF-Tissue,IHC-P,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
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