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p63 Recombinant Antibody [SC06-31] - Mouse IgG1 (Chimeric) - BSA and Azide free

Usd: 649

Specification

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Catalog# HA610429

p63 Recombinant Antibody [SC06-31] - Mouse IgG1 (Chimeric) - BSA and Azide free

Application

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

p63 Recombinant Antibody [SC06-31] - Mouse IgG1 (Chimeric) - BSA and Azide free

Antibody Type

Recombinant Chimeric Antibody

Immunogen

Recombinant protein within human p63 aa 1-250.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 77 kDa

Positive Control

A431 (Human epidermoid carcinoma skin squamous cell) cell lysate.

Conjugation

unconjugated

Clone Number

SC06-31

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IF-Cell

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:500

Target

Function

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function. While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors. Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck. The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms.

Background References

1. Wu G et al. TP63 fusions drive multicomplex enhancer rewiring, lymphomagenesis, and EZH2 dependence. Sci Transl Med. 2023 Sep

2. Cai H et al. TP63 as a modulator of ferroptosis in TP53 mutations glioblastoma. Cell Death Dis. 2025 Aug

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9H3D4 Human

SwissProt: O88898 Mouse

SwissProt: Q9JJP6 Rat

Synonyms

AIS antibody

Amplified in squamous cell carcinoma antibody

B(p51A) antibody

B(p51B) antibody

Chronic ulcerative stomatitis protein antibody

CUSP antibody

DN p63 alpha 1 antibody

DNp63 antibody

EEC3 antibody

id:ibd3516 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of p63 on different lysates with Mouse anti-p63 antibody (<a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>) at 1/5,000 dilution.<br /><br />Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate<br />Lane 2: MCF7 (Human breast cancer cell) cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 10 seconds; ECL: K1801<br /><br />Negative control: MCF7 (PMID: 17446929).<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Mouse IgG-HRP (<a href="/products/HA1006" style="font-weight: bold;text-decoration: underline;">HA1006</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 77 kDa<br />Observed band size: 63 kDa

    ☑ Relative expression (RE)

    Western blot analysis of p63 on different lysates with Mouse anti-p63 antibody (HA610429) at 1/5,000 dilution.

    Lane 1: A431 (Human epidermoid carcinoma skin squamous cell) cell lysate
    Lane 2: MCF7 (Human breast cancer cell) cell lysate

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 10 seconds; ECL: K1801

    Negative control: MCF7 (PMID: 17446929).

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA610429, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Mouse IgG-HRP (HA1006), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 77 kDa
    Observed band size: 63 kDa

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Application: Immunocytochemistry (IF-cell) <br /> <br />Species: Human <br />Sample: A431 (Human epidermoid carcinoma skin squamous cell) / MCF7 (Human breast cancer cell) <br /> <br />Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. <br />Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. <br />Antibody dilution buffer: 1% BSA in PBST. <br /> <br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/1,000, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 45 minutes at room temperature. <br />Counterstain: Beta tubulin (<a href="/products/ET1602-4" style="font-weight: bold;text-decoration: underline;">ET1602-4</a>, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue). <br /> <br />Low expression: MCF7 (PMID: 26704768).

    ☑ Relative expression (RE)

    Application: Immunocytochemistry (IF-cell)

    Species: Human
    Sample: A431 (Human epidermoid carcinoma skin squamous cell) / MCF7 (Human breast cancer cell)

    Fixation: 4% Paraformaldehyde, 15 minutes at room temperature.
    Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature.
    Antibody dilution buffer: 1% BSA in PBST.

    Primary antibody: HA610429, 1/1,000, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 45 minutes at room temperature.
    Counterstain: Beta tubulin (ET1602-4, Red), 1/200, overnight at 4℃. The nuclear counterstain was DAPI (Blue).

    Low expression: MCF7 (PMID: 26704768).

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Human <br />Tissue: Skin <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Human
    Tissue: Skin
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610429, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Mouse <br />Tissue: Skin <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Mouse
    Tissue: Skin
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610429, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunohistochemistry (IHC-P) <br /> <br />Species: Rat <br />Tissue: Skin <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes. <br /> <br />Wash buffer: 1× TBST <br />Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature. <br />Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/1,000, 1 hour at room temperature. <br />Secondary antibody: <a href="/products/HA1120" style="font-weight: bold;text-decoration: underline;">HA1120</a>, 20 minutes at room temperature.

    Application: Immunohistochemistry (IHC-P)

    Species: Rat
    Tissue: Skin
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, sodium citrate buffer (pH 6.0), high pressure for 2 minutes.

    Wash buffer: 1× TBST
    Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
    Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
    Primary antibody: HA610429, 1/1,000, 1 hour at room temperature.
    Secondary antibody: HA1120, 20 minutes at room temperature.

  • Application: Immunofluorescence (IF-tissue) <br /> <br />Species: Human <br />Tissue: Skin <br />Sample: Paraffin-embedded section <br /> <br />Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C. <br /> <br />Wash buffer: 1× TBST <br />Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature. <br />Primary antibody: <a href="/products/HA610429" style="font-weight: bold;text-decoration: underline;">HA610429</a>, 1/500, overnight at 4°C. <br />Secondary antibody: Goat Anti-Mouse IgG (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>), 1.5 hours at room temperature.

    Application: Immunofluorescence (IF-tissue)

    Species: Human
    Tissue: Skin
    Sample: Paraffin-embedded section

    Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.

    Wash buffer: 1× TBST
    Blocking: 10% normal goat serum + 1% Triton X-100 + 0.3 M Glycine in TBST, 30 minutes at room temperature.
    Primary antibody: HA610429, 1/500, overnight at 4°C.
    Secondary antibody: Goat Anti-Mouse IgG (iFluor™ 488, HA1125), 1.5 hours at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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