p63 Recombinant Rabbit Monoclonal Antibody [SC06-31] (ET1610-44)
Catalog# ET1610-44
Rabbit Monoclonal to p63
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: A431 cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane) (low expression)
Lane 3: HaCaT cell lysate (15 µg/Lane)
Lane 4: Mouse skin tissue lysate (30 µg/Lane)
Lane 5: Rat skin tissue lysate (30 µg/Lane)
Predicted band size: 77 kDa
Observed band size: 60-70 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-44) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
p63 Recombinant Rabbit Monoclonal Antibody [SC06-31] (ET1610-44)
Immunogen
Recombinant protein within human p63 aa 1-250.
Host
Rabbit
Positive Control
A431 cell lysate, MCF7 cell lysate, HaCaT cell lysate, Mouse skin tissue lysate, Rat skin tissue lysate, A431, human skin tissue, human breast tissue, human esophagus tissue, mouse esophagus tissue, rat esophagus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC06-31
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:500-1:2,000
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IF-Tissue
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1:50-1:200
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IHC-P
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1:800-1:2,000
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 77 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
p63
SYNONYMS
AIS antibody;Amplified in squamous cell carcinoma antibody;B(p51A) antibody;B(p51B) antibody;Chronic ulcerative stomatitis protein antibody;CUSP antibody;DN p63 alpha 1 antibody;DNp63 antibody;EEC3 antibody;id:ibd3516 antibody;Keratinocyte transcription factor antibody;Keratinocyte transcription factor KET antibody;KET antibody;LMS antibody;MGC115972 antibody;MGC192897 antibody;NBP antibody;OFC8 antibody;OTTHUMP00000209732 antibody;OTTHUMP00000209733 antibody;OTTHUMP00000209734 antibody;OTTHUMP00000209735 antibody;OTTHUMP00000209737 antibody;OTTHUMP00000209738 antibody;OTTHUMP00000209739 antibody;OTTHUMP00000209740 antibody;OTTHUMP00000209741 antibody;OTTHUMP00000209742 antibody;OTTHUMP00000209743 antibody;OTTHUMP00000209744 antibody;p40 antibody;p51 antibody;P51/P63 antibody;p53-related protein p63 antibody;p53CP antibody;p63 antibody;P63_HUMAN antibody;p73H antibody;p73L antibody;RHS antibody;SHFM4 antibody;TAp63alpha antibody;TP53CP antibody;TP53L antibody;TP63 antibody;TP73L antibody;Transformation related protein 63 antibody;Transformation-related protein 63 antibody;Trp53rp1 antibody;Trp63 antibody;Tumor protein 63 antibody;Tumor protein p53-competing protein antibody;Tumor protein p53-like antibody;Tumor protein p63 antibody;Tumor protein p63 deltaN isoform delta antibody;Tumor protein p73 antibody;Tumor protein p73-like antibody
SEQUENCE SIMILARITIES
Belongs to the p53 family.
TISSUE SPECIFICITY
Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues.
POST-TRANSLATIONAL MODIFICATION
May be sumoylated.; Ubiquitinated. Polyubiquitination involves WWP1 and leads to proteasomal degradation of this protein.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. In addition to p53, mammalian cells contain two p53 family members, p63 and p73, which are similar to p53 in both structure and function. While p63 can induce p53-responsive genes and apoptosis, mutation of p63 rarely results in tumors. Research investigators frequently observe amplification of the p63 gene in squamous cell carcinomas of the lung, head and neck. The p63 gene contains an alternative transcription initiation site that yields a 40 kDa δNp63 lacking the transactivation domain, and alternative splicing at the carboxy-terminus yields the α, β, and γ isoforms.
CITATIONS
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Chen, H., Hu, K., Xie, Y., Qi, Y., Li, W., He, Y., Fan, S., Liu, W., & Li, C.
CDK1 Promotes Epithelial-Mesenchymal Transition and Migration of Head and Neck Squamous Carcinoma Cells by Repressing ∆Np63α-Mediated Transcriptional Regulation
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WB
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Citation
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