p75 NGF Receptor Recombinant Antibody [PSH13-26] - Rabbit IgG (Chimeric)
Catalog# HA723532
p75 NGF Receptor Recombinant Antibody [PSH13-26] - Rabbit IgG (Chimeric)
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IHC-Fr
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IF-Cell
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Mouse
-
unconjugated
Overview
Product Name
p75 NGF Receptor Recombinant Antibody [PSH13-26] - Rabbit IgG (Chimeric)
Antibody Type
Recombinant Chimeric Antibody
Species Reactivity
Mouse
Validated Applications
IHC-Fr, IF-Cell
Target Molecular Weight
Predicted band size: 46 kDa
Conjugation
unconjugated
Clone Number
PSH13-26
Reactivity Data
| IHC-Fr | IF-Cell | |
|---|---|---|
| Mouse |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.
Purification Method
Protein A affinity purified.
Application Dilution
-
IHC-Fr
-
1:500
-
IF-Cell
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1:50-1:100
Target
Function
The p75 neurotrophin receptor (p75NTR) was first identified in 1973 as the low-affinity nerve growth factor receptor (LNGFR) before discovery that p75NTR bound other neurotrophins equally well as nerve growth factor. p75NTR is a neurotrophic factor receptor. Neurotrophic factor receptors bind Neurotrophins including Nerve growth factor, Neurotrophin-3, Brain-derived neurotrophic factor, and Neurotrophin-4. All neurotrophins bind to p75NTR. This also includes the immature pro-neurotrophin forms. Neurotrophic factor receptors, including p75NTR, are responsible for ensuring a proper density to target ratio of developing neurons, refining broader maps in development into precise connections. p75NTR is involved in pathways that promote neuronal survival and neuronal death.
Background References
1. Bruno F et al. The Nerve Growth Factor Receptor (NGFR/p75(NTR)): A Major Player in Alzheimer\'s Disease. Int J Mol Sci. 2023 Feb
2. Castellini C et al. The Effect of Interaction NGF/p75(NTR) in Sperm Cells: A Rabbit Model. Cells. 2022 Mar
Subcellular Location
Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine.
UNIPROT
Synonyms
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
ExpandImages
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Application: IHC-Fr
Species: Mouse
Site: brain
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required -
Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rabbit anti-p75 NGF Receptor antibody (HA723532) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p75 NGF Receptor antibody (HA723532) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (HA601187 , red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"