Phospho-STAT3 (Y705) Recombinant Rabbit Monoclonal Antibody [SZ43-01] (ET1603-40)
Catalog# ET1603-40
Rabbit Monoclonal to Phospho-STAT3 (Y705)
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes whole cell lysate
Lane 5: A431 whole cell lysate
Lane 6: A431 treated with 100ng/mL EGF for 30 minutes whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 88 kDa
Exposure time: 35 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1603-40) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Phospho-STAT3 (Y705) Recombinant Rabbit Monoclonal Antibody [SZ43-01] (ET1603-40)
Immunogen
Synthetic phospho-peptide corresponding to residues surrounding tyr705 of human stat3.
Host
Rabbit
Modification
Phospho
Modification Site
Y705
Positive Control
HeLa treated with 50ng/mL IFN alpha 1 for 30 minutes whole cell lysate, Jurkat treated with 50ng/mL IFN alpha 1 for 30 minutes whole cell lysate, A431 treated with 100ng/mL EGF for 30 minutes whole cell lysate, human lung tissue, Hela cell lysate, SK-Br-3 cell lysate, human kidney tissue, mouse skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SZ43-01
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:500
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 88 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Phospho-STAT3 (Y705)
SYNONYMS
1110034C02Rik antibody;Acute Phase Response Factor antibody;Acute-phase response factor antibody;ADMIO antibody;APRF antibody;AW109958 antibody;DNA binding protein APRF antibody;FLJ20882 antibody;HIES antibody;MGC16063 antibody;Signal transducer and activator of transcription 3 (acute phase response factor) antibody;Signal transducer and activator of transcription 3 antibody;STAT 3 antibody;Stat3 antibody;STAT3_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the transcription factor STAT family.
TISSUE SPECIFICITY
Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
POST-TRANSLATIONAL MODIFICATION
Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.; Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.; Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity. Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated.; (Microbial infection) Phosphorylated on Tyr-705 in the presence of S.typhimurium SarA.; S-palmitoylated by ZDHHC19 in SH2 putative lipid-binding pockets, leading to homodimerization. Nuclear STAT3 is highly palmitoylated (about 75%) compared with cytoplasmic STAT3 (about 20%).; S-stearoylated, probably by ZDHHC19.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF, LEP and other growth factors. Once activated, recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target gene. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Acts as a regulator of inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells (Treg): deacetylation and oxidation of lysine residues by LOXL3, leads to disrupt STAT3 dimerization and inhibit its transcription activity. Involved in cell cycle regulation by inducing the expression of key genes for the progression from G1 to S phase, such as CCND1. Mediates the effects of LEP on melanocortin production, body energy homeostasis and lactation. May play an apoptotic role by transctivating BIRC5 expression under LEP activation. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays a crucial role in basal beta cell functions, such as regulation of insulin secretion.
CITATIONS
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Zhaolu Kong
STAT3 enhances radiation-induced tumor migration,invasion and stem-like properties of bladder cancer
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WB
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human
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Citation
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Que, H., Hong, W., Lan, T., Zeng, H., Chen, L., Wan, D., Bi, Z., Ren, W., Luo, M., Yang, J., He, C., Zhong, A., & Wei, X.
Tripterin liposome relieves severe acute respiratory syndrome as a potent COVID-19 treatment
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WB
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Mouse
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Monkey
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Citation
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Osteoclast-Derived Exosomal miR-5134-5p Interferes with Alveolar Bone Homeostasis by Targeting the JAK2/STAT3 Axis
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WB
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Mouse
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Citation
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-
Phellopterin alleviates atopic dermatitis-like inflammation and suppresses IL-4-induced STAT3 activation in keratinocytes
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IHC
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WB
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Mouse/Human
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Citation
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Jinge Dou,et al
P. Gingivalis induce macrophage polarization by regulating hepcidin expression in chronic apical periodontitis
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WB
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Human
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Citation
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Bi-Wen Peng
TRPV1 mediates astrocyte activation and interleukin-1β release induced by hypoxic ischemia (HI)
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WB
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human
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Citation
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Min Hou,et al
Naringenin alleviates intestinal ischemia/reperfusion injury by inhibiting ferroptosis via targeting YAP/STAT3 signaling axis
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Nuclear translocation of PKM2 mediates keratinocyte metabolic reprogramming in psoriasis
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WB
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Human
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Citation
-
-
Daisong Li,et al
Integrated metabolomics and network pharmacology to reveal the mechanisms of Shexiang Baoxin pill against atherosclerosis
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WB
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Mouse
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Citation
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-
Liu, Y., Hao, Y., Zhao, H., Zhang, Y., Cheng, D., Zhao, L., Peng, Y., Lu, Y., & Li, Y.
PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells
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WB
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Human
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Citation
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-
Tang Xueqian,et al
α7nAChR-mediated astrocytic activation: a novel mechanism of Xiongzhi Dilong decoction in ameliorating chronic migraine
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WB
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Rat
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Citation
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Lihua Zhang, Weibo Shi, Jingmin Liu, Ke Chen, Guowei Zhang, Shengnan Zhang, Bin Cong, Yingmin Li
Interleukin 6 (IL-6) Regulates GABAA Receptors in the Dorsomedial Hypothalamus Nucleus (DMH) through Activation of the JAK/STAT Pathway to Affect Heart Rate Variability in Stressed Rats
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WB
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Rat
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Citation
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Acute myeloid leukemia (AML)‐derived mesenchymal stem cells induce chemoresistance and epithelial–mesenchymal transition‐like program in AML through IL‐6/JAK2/STAT3 signaling
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WB
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Human
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Citation
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Dr Yuan-Zhi Yuan
Platelet-derived growth factor-BB promotes proliferation and migration of retinal microvascular pericytes by up-regulating the expression of C-X-C chemokine receptor types 4
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WB
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human
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Citation
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Wang Yingying,et al
Linderae Radix extract attenuates ulcerative colitis by inhibiting the JAK/STAT signaling pathway
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WB
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IHC
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Mouse
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Citation
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Songhua Hu
Receptor and signaling pathway involved in bovine lymphocyte activation by Atractylodis macrocephalae polysaccharides
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WB
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cow
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Citation
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Liu, Z. Y., Lan, T., Tang, F., He, Y. Z., Liu, J. S., Yang, J. Z., Chen, X., Wang, Z. F., & Li, Z. Q.
ZDHHC15 promotes glioma malignancy and acts as a novel prognostic biomarker for patients with glioma
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WB
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Human
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Citation
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Deng, L., Zhang, X., Xiang, X., Xiong, R., Xiao, D., Chen, Z., Liu, K., & Feng, G.
NANOG Promotes Cell Proliferation, Invasion, and Stemness via IL-6/STAT3 Signaling in Esophageal Squamous Carcinoma
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WB
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Human
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Citation
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Fan Chengcheng,et al
Natural Product-Inspired Discovery of Naphthoquinone-Furo-Piperidine Derivatives as Novel STAT3 Inhibitors for the Treatment of Triple-Negative Breast Cancer
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WB
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Human
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Citation
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-
Novel synthetic 4-chlorobenzoyl berbamine inhibits c-Myc expression and induces apoptosis of diffuse large B cell lymphoma cells.
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WB
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Human
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Citation
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Xiao, X., Zou, S., & Chen, J.
Cyclic tensile force modifies calvarial osteoblast function via the interplay between ERK1/2 and STAT3
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WB
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Mouse
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Citation
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-
Shi Yajing,et al
Ursolic acid improves necroptosis via STAT3 signaling in intestinal ischemia/reperfusion injury
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WB
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Mouse
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Citation
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-
Yang Boshen,et al
Ruxolitinib-based senomorphic therapy mitigates cardiomyocyte senescence in septic cardiomyopathy by inhibiting the JAK2/STAT3 signaling pathway
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WB
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Mouse
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Citation
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-
Renhao Geng,et al
Punicalagin Inhibits African Swine Fever Virus Replication by Targeting Early Viral Stages and Modulating Inflammatory Pathways
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WB
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Sus Scrofa
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Citation
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Filamin A is overexpressed in non-alcoholic steatohepatitis and contributes to the progression of inflammation and fibrosis
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WB
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Mouse
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Citation
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-
Xianlei Han,et al
Network pharmacology and phytochemical composition combined with validation in vivo and in vitro reveal the mechanism of platycodonis radix ameliorating PM2.5-induced acute lung injury
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WB
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Mouse
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Citation
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Jian-Li Jiang,Hong-Yong Cu,Zhi-Nan Chen
Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis. Cellular oncology (Dordrecht), 42(4), 537–554.
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WB
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CO-IP
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IHC
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IF
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Human hepatocellular carcinoma cell
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Citation
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Autophagy inhibition enhances anti‐pituitary adenoma effect of tetrandrine
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WB
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rat
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Citation
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-
Chen, X., Dou, J., Fu, Z., Qiu, Y., Zou, L., Huang, D., & Tan, X.
Macrophage M1 polarization mediated via the IL-6/STAT3 pathway contributes to apical periodontitis induced by Porphyromonas gingivalis
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WB
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Human
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Citation
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Ruiqin Hu,et al
bmp10 maintains cardiac function by regulating iron homeostasis
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WB
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Zebrafish
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Citation
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Resveratrol decreases CD45 CD206 subtype macrophages in LPS-induced murine acute lung injury by SOCS3 signalling pathway.
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WB
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mouse
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Citation
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-
Hu, Y., Feng, L., Jiang, W., Wu, P., Liu, Y., Kuang, S., Tang, L., & Zhou, X.
Lysine deficiency impaired growth performance and immune response and aggravated inflammatory response of the skin, spleen and head kidney in grown-up grass carp (Ctenopharyngodon idella). Animal nutrition (Zhongguo xu mu shou yi xue hui), 7(2), 556–568.
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WB
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Grass carp
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Citation
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Song Meiying,et al
Pathogenic Th17 cell-mediated liver fibrosis contributes to resistance to PD-L1 antibody immunotherapy in hepatocellular carcinoma
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WB
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Mouse
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Citation
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-
Li Weijing,et al
Dexmedetomidine hydrochloride plus sufentanil citrate inhibits glucose metabolism and epithelial‑mesenchymal transition in human esophageal squamous carcinoma KYSE30 cells by modulating the JAK/STAT3/HIF‑1α axis
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WB
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Human
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Citation
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-
Exosomes of oral squamous cell carcinoma cells containing miR-181a-3p induce muscle cell atrophy and apoptosis by transmissible endoplasmic reticulum stress signaling
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WB
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Mouse
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Citation
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-
Zhang J, Wang W, Liang S, et al
Butyrate induces STAT3/HIF-1α/IL-22 signaling via GPCR and HDAC3 inhibition to activate autophagy in head kidney macrophages from turbot (Scophthalmus maximus L.)
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WB
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Channa punctatus
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Citation
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