Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] (ET1702-06)
Catalog# ET1702-06
Rabbit Monoclonal to Transferrin Receptor (CD71)
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WB
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IP
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FC
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: SW480 cell lysate
Lane 4: U-87 MG cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: Mouse spleen tissue lysate
Lane 7: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 85 kDa
Observed band size: 100 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-06) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Transferrin Receptor (CD71) Recombinant Rabbit Monoclonal Antibody [JF0956] (ET1702-06)
Immunogen
Synthetic peptide within human cd71 aa 22-60 / 760.
Host
Rabbit
Positive Control
HeLa cell lysate, K-562 cell lysate, SW480 cell lysate, U-87 MG cell lysate, RAW264.7 cell lysate, mouse spleen tissue lysate, rat spleen tissue lysate, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF0956
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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FC
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1:50-1:100
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 85 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Transferrin Receptor (CD71)
SYNONYMS
CD 71 antibody;CD71 antibody;CD71 antigen antibody;IMD46 antibody;OTTHUMP00000208523 antibody;OTTHUMP00000208524 antibody;OTTHUMP00000208525 antibody;p90 antibody;sTfR antibody;T9 antibody;TFR 1 antibody;TfR antibody;TfR1 antibody;TFR1_HUMAN antibody;TFRC antibody;TR antibody;Transferrin receptor (p90 CD71) antibody;Transferrin receptor protein 1, serum form antibody;Trfr antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase M28 family. M28B subfamily.
POST-TRANSLATIONAL MODIFICATION
N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.; Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).; Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
SUBCELLULAR LOCATION
Cell membrane, Melanosome, Secreted.
FUNCTION
CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. CD71 binds to two molecules of transferrin and a serum iron-transport protein, and directs the cellular uptake of iron via receptor-mediated endocytosis. CD71 is expressed, typically at high levels, on all proliferating cells, reticulocytes and erythroid precursors. It is not expressed on resting leukocytes, but is upregulated upon activation of lymphocytes, monocytes and macrophages. CD71 is also found on most dividing cells and on brain endothelium. A second transferrin receptor, TFR2, also mediates the uptake of transferrin-bound iron. TFR2 is a two-subunit homodimer and is highly expressed in liver as well as in hepatocytes and erythroid precursors. Mutations in the TFR2 gene result in hereditary hemochromatosis type III (HFE3), an iron overloading disorder predominant in Caucasians.
CITATIONS
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Zhao Jingyuan,et al
Heme oxygenase-1 is involved in the erastin-induced ferroptosis in acute myeloid leukemia cells
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WB
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Human
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Citation
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Ren, Q., Wang, B., Guo, F., Huang, R., Tan, Z., Ma, L., & Fu, P.
Natural Flavonoid Pectolinarigenin Alleviated Hyperuricemic Nephropathy via Suppressing TGFβ/SMAD3 and JAK2/STAT3 Signaling Pathways
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WB
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Mouse
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Citation
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Yuejia Li,et al
Zinc Attenuates Bisphenol A-Induced Reproductive Toxicity in Male Mice by Inhibiting Ferroptosis and Apoptosis Through Improving Zinc Homeostasis
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WB
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Mouse
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Citation
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Weichu Sun,et al
Sulforaphane inhibits multiple myeloma cell-induced osteoclast differentiation and macrophage proliferation by elevating ferroportin1
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WB
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Human
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Citation
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He Yijing,et al
Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation
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WB
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Porcine
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Citation
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Chengwei Wu,et al
NPR1 promotes cisplatin resistance by inhibiting PARL-mediated mitophagy-dependent ferroptosis in gastric cancer
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WB
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Mouse
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Citation
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Ruijian Zhuge,et al
Ferritinophagy is involved in hexavalent chromium-induced ferroptosis in Sertoli cells
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WB
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Rat
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Citation
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Wang Rui,et al
Screening for ferroptosis genes related to endometrial carcinoma and predicting of targeted drugs based on bioinformatics
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IHC
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Human
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Citation
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Shikonin and cisplatin synergistically overcome cisplatin resistance of ovarian cancer by inducing ferroptosis via upregulation of HMOX1 to promote Fe2+ accumulation
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WB
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Human
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Citation
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Liang, J., Shen, Y., Wang, Y., Huang, Y., Wang, J., Zhu, Q., Tong, G., Yu, K., Cao, W., Wang, Q., Li, Y., & Zhao, Y.
Ferroptosis participates in neuron damage in experimental cerebral malaria and is partially induced by activated CD8 + T cells
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WB
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Mouse
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Citation
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Chen Ying,et al
Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis
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WB
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Mouse
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Citation
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Sun, Y., He, Y., Tong, J., Liu, D., Zhang, H., He, T., & Bi, Y.
All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis
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WB
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Human
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Citation
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Jin, L., Yu, B., Wang, H., Shi, L., Yang, J., Wu, L., Gao, C., Pan, H., Han, F., Lin, W., Lai, E. Y., Wang, Y. F., & Yang, Y.
STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury
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WB
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Mouse
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Citation
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