Tri-Methyl-Histone H4 (K20) Recombinant Rabbit Monoclonal Antibody [ST044-07] (ET1609-16)

UNIPROT #

P62805

PROTEIN NAME

Tri-Methyl-Histone H4 (K20)

SYNONYMS

Histone gene cluster 1, H4A antibody;Histone gene cluster 2, H4 antibody;dJ160A22.1 antibody;dJ160A22.2 antibody;dJ221C16.1 antibody;dJ221C16.9 antibody;FO108 antibody;H4 antibody;H4 histone family, member A antibody;H4 histone family, member B antibody;H4 histone family, member C antibody;H4 histone family, member D antibody;H4 histone family, member E antibody;H4 histone family, member G antibody;H4 histone family, member H antibody;H4 histone family, member I antibody;H4 histone family, member J antibody;H4 histone family, member K antibody;H4 histone family, member M antibody;H4 histone family, member N antibody;H4 histone, family 2 antibody;H4/A antibody;H4/B antibody;H4/C antibody;H4/D antibody;H4/E antibody;H4/G antibody;H4/H antibody;H4/I antibody;H4/J antibody;H4/K antibody;H4/M antibody;H4/N antibody;H4/O antibody;H4/p antibody;H4_HUMAN antibody;H4F2 antibody;H4F2iii antibody;H4F2iv antibody;H4FA antibody;H4FB antibody;H4FC antibody;H4FD antibody;H4FE antibody;H4FG antibody;H4FH antibody;H4FI antibody;H4FJ antibody;H4FK antibody;HIST1 cluster, H4A antibody;HIST1 cluster, H4B antibody;HIST1 cluster, H4D antibody;HIST2H4 antibody;Hist4 cluster, H4 antibody;Hist4h4 antibody;histone 1, H4a antibody;histone 1, H4c antibody;histone 1, H4d antibody;histone 1, H4f antibody;histone 1, H4h antibody;histone 1, H4i antibody;histone 1, H4j antibody;histone 1, H4k antibody;histone 1, H4l antibody;histone 2, H4a antibody;histone 2, H4b antibody;Histone 4 family, member M antibody;histone 4, H4 antibody;histone cluster 1, H4 antibody;histone cluster 1, H4a antibody;histone cluster 1, H4b antibody;histone cluster 1, H4c antibody;histone cluster 1, H4d antibody;histone cluster 1, H4e antibody;histone cluster 1, H4f antibody;histone cluster 1, H4h antibody;histone cluster 1, H4i antibody;histone cluster 1, H4j antibody;histone cluster 1, H4k antibody;histone cluster 1, H4l antibody;histone cluster 2, H4a antibody;histone cluster 2, H4b antibody;histone cluster 4, H4 antibody;Histone family, member A antibody;Histone family, member B antibody;Histone family, member D antibody;Histone family, member H antibody;Histone family, member I antibody;Histone family, member L antibody;Histone gene cluster 1, H4 antibody;Histone gene cluster 1, H4D antibody;Histone gene cluster 1, H4E antibody;Histone gene cluster 1, H4K antibody;Histone gene cluster 4, H4 antibody;Histone gene cluster 4, H4 histone antibody;Histone H4 antibody;histone IV, family 2 antibody;H4K20me3 antibody

SEQUENCE SIMILARITIES

Belongs to the histone H4 family.

POST-TRANSLATIONAL MODIFICATION

Acetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.; Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.; Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.; Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Dimethylation and trimethylation is performed by KMT5B and KMT5C and induces gene silencing (By similarity).; Phosphorylated by PAK2 at Ser-48 (H4S47ph). This phosphorylation increases the association of H3.3-H4 with the histone chaperone HIRA, thus promoting nucleosome assembly of H3.3-H4 and inhibiting nucleosome assembly of H3.1-H4.; Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).; Sumoylated, which is associated with transcriptional repression.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.; Butyrylation of histones marks active promoters and competes with histone acetylation.; Glutarylation at Lys-92 (H4K91glu) destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes.

SUBCELLULAR LOCATION

Nucleus, Chromosome.

FUNCTION

Eukaryotic histones are basic and water soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fibers. Two molecules of each of the four core histones (H2A, H2B, H3 and H4) form the octamer, which is comprised of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Human and mouse Histone H4 are subject to trimethylation at Lys 20, a modification that may be necessary for select DNA transactions or chromatin state transitions.