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CD34 Rabbit Polyclonal Antibody

Usd: 315

Specification

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Catalog# 0108-2

CD34 Rabbit Polyclonal Antibody

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Zebrafish

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD34 Rabbit Polyclonal Antibody

Antibody Type

Rabbit Polyclonal Antibody

Immunogen

Synthetic peptide within human CD34 aa 336-385.

Species Reactivity

Human, Mouse, Zebrafish

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 41 kDa

Positive Control

Human brain, mouse brain, mouse testis, Mouse thymus, mouse spleen, Jurkat, TF-1, F9, human embryonic stem cell, human tonsil tissue

Conjugation

unconjugated

RRID

AB_3096758

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Immunogen affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:3,000

Target

Function

CD34 is a transmembrane phosphoglycoprotein protein encoded by the CD34 gene in humans, mice, rats and other species. The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show expression on early haematopoietic and vascular-associated progenitor cells. However, little is known about its exact function. CD34 is also an important adhesion molecule and is required for T cells to enter lymph nodes. It is expressed on lymph node endothelia, whereas the L-selectin to which it binds is on the T cell. Conversely, under other circumstances CD34 has been shown to act as molecular "Teflon" and block mast cell, eosinophil and dendritic cell precursor adhesion, and to facilitate opening of vascular lumina. Finally, recent data suggest CD34 may also play a more selective role in chemokine-dependent migration of eosinophils and dendritic cell precursors. Regardless of its mode of action, under all circumstances CD34, and its relatives podocalyxin and endoglycan, facilitates cell migration.

Background References

1. Freund D., Wiebe G.J., Ehninger G., Corbeil D.; "Sequence analysis of the human CD34 antigen."; Submitted (JUN-2002) to the EMBL/GenBank/DDBJ databases.

2. The MGC Project Team;"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

3. Sutherland D.R., Watt S.M., Dowden G., Karhi K., Baker M.A., Greaves M.F., Smart J.E.;"Structural and partial amino acid sequence analysis of the human hemopoietic progenitor cell antigen CD34.";Leukemia 2:793-803(1988).

Sequence Similarity

Belongs to the CD34 family.

Tissue Specificity

Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

Post-translational Modification

Highly glycosylated.; Phosphorylated on serine residues by PKC.

Subcellular Location

Cell membrane

UNIPROT

SwissProt: P28906 Human

SwissProt: Q64314 Mouse

Synonyms

CD34 antibody

CD34 antigen antibody

CD34 molecule antibody

CD34_HUMAN antibody

Cluster designation 34 antibody

Hematopoietic progenitor cell antigen CD34 antibody

HPCA1 antibody

Mucosialin antibody

OTTHUMP00000034733 antibody

OTTHUMP00000034734 antibody

Images

  • Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1,000 dilution.<br /><br />Positive control: <br />Lane 1: Human brain lysate<br />Lane 2: Mouse brain lysate<br />Lane 3: Mouse testis lysate<br />Lane 4: Mouse thymus lysate<br />Lane 5: Mouse spleen lysate<br />Lane 6:Jurkat lysate<br />Lane 7: TF-1 lysate<br />Lane 8: F9 lysate<br /><br />Predicted band size: 41 kDa<br />Observed band size: 90 kDa

    Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1,000 dilution.

    Positive control:
    Lane 1: Human brain lysate
    Lane 2: Mouse brain lysate
    Lane 3: Mouse testis lysate
    Lane 4: Mouse thymus lysate
    Lane 5: Mouse spleen lysate
    Lane 6:Jurkat lysate
    Lane 7: TF-1 lysate
    Lane 8: F9 lysate

    Predicted band size: 41 kDa
    Observed band size: 90 kDa

  • ICC staining of CD34 in hES cells (green). Formalin fixed cells were permeabilized with 0.25% Triton X100/PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (<a href="/products/0108-2" style="font-weight: bold;text-decoration: underline;">0108-2</a>, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor&reg;488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

    ICC staining of CD34 in hES cells (green). Formalin fixed cells were permeabilized with 0.25% Triton X100/PBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (0108-2, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 conjugate-Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/0108-2" style="font-weight: bold;text-decoration: underline;">0108-2</a>, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD34 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0108-2, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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