SCA-1 Rabbit Polyclonal Antibody

Function

Sca-1 stands for "Stem cells antigen-1" (official gene symbol: Ly6a). It consist of 18-kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein (GPI-AP) of the LY6 gene family. It is the common biological marker used to identify hematopoitic stem cell (HSC) along with other markers. Sca-1 has a regenerative role in cardiac repair: Host cells with specific Sca-1+CD31− markers arise upon myocardial infarction, with evidence of expression of Sca-1 protein. Sca-1 plays a role in hematopoietic progenitor/stem cell lineage fate and c-kit expression. Sca-1+ HSCs can be found in the adult bone marrow, fetal liver and mobilized peripheral blood and spleen within the adult animal. An anti-Sca-1 Ab is frequently used in combination with negative selection for expression of a number of cell surface markers characteristic of differentiated cells of hematolymphoid lineages (Lin-) to identify and isolate murine HSCs.

Background References

1. Khan K., Dad Lindwall G., Maher S.E., Bothwell A.L.M.; "Characterization of promoter elements of an interferon-inducible Ly-6E/A differentiation antigen, which is expressed on activated T cells and hematopoietic stem cells."; Mol. Cell. Biol. 10:5150-5159(1990).

2. van de Rijn M., Heimfeld S., Spangrude G.J., Weissman I.L.; "Mouse hematopoietic stem-cell antigen Sca-1 is a member of the Ly-6 antigen family."; Proc. Natl. Acad. Sci. U.S.A. 86:4634-4638(1989).

3. van Bragt M.P., Ciliberti N., Stanford W.L., de Rooij D.G., van Pelt A.M.; "LY6A/E (SCA-1) expression in the mouse testis."; Biol. Reprod. 73:634-638(2005).

Tissue Specificity

Widely expressed.

Post-translational Modification

O-glycosylated. Not N-glycosylated.; Not phosphorylated.

Subcellular Location

Cell membrane

UNIPROT

SwissProt: P05533 Mouse

Synonyms