CD10 Rabbit Polyclonal Antibody
Catalog# 0805-4
CD10 Rabbit Polyclonal Antibody
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WB
-
IHC-P
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Human
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Mouse
-
Rat
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unconjugated
Overview
Product Name
CD10 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide within Human CD10 aa 205-254 / 750.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 86 kDa
Positive Control
LNCaP cell lysate, Ramos cell lysate, Raji cell lysate, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
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1:2,000
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IHC-P
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1:50-1:200
Target
Function
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Background References
1. Morisaki N et al. Neprilysin is identical to skin fibroblast elastase: its role in skin aging and UV responses. J Biol Chem 285:39819-39827 (2010).
2. Auer-Grumbach M et al. Rare variants in MME, encoding metalloprotease neprilysin, are linked to late-onset autosomal-dominant axonal polyneuropathies. Am J Hum Genet 99:607-623 (2016).
Sequence Similarity
Belongs to the peptidase M13 family.
Post-translational Modification
Myristoylation is a determinant of membrane targeting.; Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity.
Subcellular Location
Cell membrane.
Synonyms
Atriopeptidase antibody
CALLA antibody
CD10 antibody
CD10 antigen antibody
Common acute lymphocytic leukemia antigen antibody
DKFZp686O16152 antibody
EC 3.4.24.11 antibody
Enkephalinase antibody
EPN antibody
Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of CD10 on different lysates with Rabbit anti-CD10 antibody (0805-4) at 1/2,000 dilution.
Lane 1: LNCaP cell lysate
Lane 2: Ramos cell lysate
Lane 3: HT-29 cell lysate (negative)
Lane 4: Raji cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 86 kDa
Observed band size: 100 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (0805-4) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CD10 antibody. Counter stained with hematoxylin.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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