CD271 Rabbit Polyclonal Antibody
Catalog# 1003-9
CD271 Rabbit Polyclonal Antibody
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WB
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IF-Cell
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IHC-P
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Mouse
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Rat
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unconjugated
Overview
Product Name
CD271 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Mouse CD271 aa 91-140 / 427 mouse.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
SW480 cell lysate,Neuro-2a cell lysate, PC-12 cell lysate,mouse brain, rat brain,L6.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
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WB
-
1:2,000-1:5,000
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IF-Cell
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1:50
Target
Function
NGFR p75 or CD271 is a 75kD transmembrane glycoprotein consisting of an extracellular domain which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain, and a cytoplasmic domain. NGFR p75 displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, it recognizes neurotrophins and transmits trophic signals into the cell. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system.
Background References
1. "Low-affinity nerve-growth factor receptor (p75NTR) can serve as a receptor for rabies virus."Tuffereau C., Benejean J., Blondel D., Kieffer B., Flamand A.EMBO J. 17:7250-7259(1998)
2. "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.J. Proteome Res. 7:5314-5326(2008)
Tissue Specificity
Detected in Schwann cells. Detected in embryonic brain, in hippocampus neurons (at protein level). Detected in brain and spinal cord.
Post-translational Modification
N-glycosylated. O-glycosylated.; Phosphorylated on serine residues.
Subcellular Location
Cell membrane, Perikaryon, Cell projection, growth cone, Cell projection, dendritic spine.
Synonyms
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
ExpandImages
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Western blot analysis of CD271 on different lysates with Rabbit anti-CD271 antibody (1003-9) at 1/5,000 dilution.
Lane 1: SW480 (Human colorectal zdenocarcinoma cell) cell lysate(15 µg/Lane)
Lane 2: Neuro-2a (Mouse brain neuroblastoma cell) cell lysate(15 µg/Lane)
Lane 3: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate(15 µg/Lane)
Lane 4: mouse brain tissue lysate(30 µg/Lane)
Lane 5: rat brain tissue lysate(30 µg/Lane)
Exposure time: 20 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: 1003-9, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa -
Immunocytochemistry analysis of L6 cells labeling CD271 with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Alternative Products
p75 NGF Receptor Recombinant Rabbit Monoclonal Antibody [SA39-02]
Application: WB,IF-Cell,IF-Tissue,IHC-P,IP,FC,IHC-Fr
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
p75 NGF Receptor Recombinant Rat Monoclonal Antibody [PSH13-26]
Application: IHC-Fr,WB,IF-Cell
Reactivity: Mouse,Rat
Conjugate: unconjugated
