CD271 Rabbit Polyclonal Antibody
Catalog# 1003-9
CD271 Rabbit Polyclonal Antibody
-
WB
-
IF-Cell
-
IHC-P
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
CD271 Rabbit Polyclonal Antibody
Antibody Type
Rabbit Polyclonal Antibody
Immunogen
Synthetic peptide corresponding to Mouse CD271 aa 91-140 / 427 mouse.
Species Reactivity
Mouse, Rat
Validated Applications
WB, IF-Cell, IHC-P
Molecular Weight
Predicted band size: 46 kDa
Positive Control
F9 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, L6.
Conjugation
unconjugated
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Immunogen affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
-
IF-Cell
-
1:50
Target
Function
NGFR p75 or CD271 is a 75kD transmembrane glycoprotein consisting of an extracellular domain which contains four cysteine rich domains responsible for ligand binding, a transmembrane domain, and a cytoplasmic domain. NGFR p75 displays paradoxical functions when acting alone or with other receptor proteins. Working in concert with Trk receptors, it recognizes neurotrophins and transmits trophic signals into the cell. NGFR p75 is necessary for regulating neuronal growth, migration, differentiation and cell death during development of the central and peripheral nervous system.
Background References
1. "Low-affinity nerve-growth factor receptor (p75NTR) can serve as a receptor for rabies virus."Tuffereau C., Benejean J., Blondel D., Kieffer B., Flamand A.EMBO J. 17:7250-7259(1998)
2. "Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry."Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M.J. Proteome Res. 7:5314-5326(2008)
Tissue Specificity
Detected in Schwann cells. Detected in embryonic brain, in hippocampus neurons (at protein level). Detected in brain and spinal cord.
Post-translational Modification
N-glycosylated. O-glycosylated.; Phosphorylated on serine residues.
Subcellular Location
Cell membrane, Perikaryon, Cell projection, growth cone, Cell projection, dendritic spine.
Synonyms
CD271 antibody
CD271 antigen antibody
Gp80 LNGFR antibody
Gp80-LNGFR antibody
Low affinity nerve growth factor receptor antibody
Low affinity neurotrophin receptor p75NTR antibody
Low-affinity nerve growth factor receptor antibody
Nerve growth factor receptor antibody
Nerve growth factor receptor TNFR superfamily member 16 antibody
NGF receptor antibody
ExpandImages
-
Western blot analysis of CD271 on different lysates with Rabbit anti-CD271 antibody (1003-9) at 1/1,000 dilution.
Lane 1: F9 cell lysate
Lane 2: PC-12 cell lysate
Lane 3: RAW264.7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 55 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (1003-9) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of L6 cells labeling CD271 with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-CD271 antibody (1003-9) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"