Antithrombin III / ATIII Recombinant Rabbit Monoclonal Antibody [PSH12-13]

Western blot analysis of Antithrombin III / ATIII on different lysates with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/5,000 dilution.

Lane 1: Mouse liver tissue lysate (40 µg/Lane)
Lane 2: Mouse testis tissue lysate (40 µg/Lane)
Lane 3: Rat testis tissue lysate (40 µg/Lane)
Lane 4: Human plasma tissue lysate (40 µg/Lane)

Predicted band size: 53 kDa
Observed band size: 53 kDa

Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723429) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis of Antithrombin III / ATIII on different lysates with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/5,000 dilution.

Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 2: Mouse liver tissue lysate
Lane 3: Mouse testis tissue lysate
Lane 4: Rat liver tissue lysate
Lane 5: Rat testis tissue lysate

Lysates/proteins at 20 µg/Lane.
Exposure time: 30 seconds; ECL: K1801

Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA723429, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

Predicted band size: 53 kDa
Observed band size: 53 kDa

Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723429) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723429) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Immunohistochemical analysis of paraffin-embedded mouse brain tissue (positive location is mainly in plasma) with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723429) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723429) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

☑ Relative expression (RE)

Immunohistochemical analysis of paraffin-embedded rat brain tissue (positive location is mainly in plasma) with Rabbit anti-Antithrombin III / ATIII antibody (HA723429) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (HA723429) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.