Biotin Conjugated Human Antithrombin III / ATIII Recombinant Rabbit Monoclonal Antibody [PSH14-24] - Detector
Overview
Product Name
Biotin Conjugated Human Antithrombin III / ATIII Recombinant Rabbit Monoclonal Antibody [PSH14-24] - Detector
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Antithrombin III aa 33-464 (HA210985).
Species Reactivity
Human
Validated Applications
ELISA(Det), ELISA
Positive Control
Recombinant Human Antithrombin III protein (HA210985).
Conjugation
Biotin
Clone Number
PSH14-24
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.05% Proclean 300.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH14-23] to Human Antithrombin III antibody (Capture) (HA723612) and Recombinant Human Antithrombin III protein (HA210985) as the standard. The reference range value is 39.1-10,000 pg/ml.
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ELISA
-
Use at an assay dependent concentration.
Target
Function
The protein encoded by this gene, antithrombin III, is a plasma protease inhibitor and a member of the serpin superfamily. This protein inhibits thrombin as well as other activated serine proteases of the coagulation system, and it regulates the blood coagulation cascade. The protein includes two functional domains: the heparin binding-domain at the N-terminus of the mature protein, and the reactive site domain at the C-terminus. The inhibitory activity is enhanced by the presence of heparin. Numerous mutations have been identified for this gene, many of which are known to cause antithrombin-III deficiency which constitutes a strong risk factor for thrombosis. A reduction in the serum level of this protein is associated with severe cases of Coronavirus Disease 19 (COVID-19).
Background References
1. Corral J., Huntington J.A., Gonzalez-Conejero R., Mushunje A., Navarro M., Marco P., Vicente V., Carrell R.W. Mutations in the shutter region of antithrombin result in formation of disulfide-linked dimers and severe venous thrombosis. J. Thromb. Haemost. 2:931-939 (2004)
2. Szabo R., Netzel-Arnett S., Hobson J.P., Antalis T.M., Bugge T.H. Matriptase-3 is a novel phylogenetically preserved membrane-anchored serine protease with broad serpin reactivity. Biochem. J. 390:231-242 (2005)
Subcellular Location
Secreted.
UNIPROT
Synonyms
ANT3_HUMAN antibody
Antithrombin antibody
Antithrombin III antibody
Antithrombin-III antibody
AntithrombinIII antibody
AT 3 antibody
AT III antibody
AT3 antibody
AT3D antibody
ATIII antibody
ExpandImages
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![Sandwich ELISA analysis of Human Antithrombin III matched pair antibodies<br /><br />Capture: <a href="/products/HA723612" style="font-weight: bold;text-decoration: underline;">HA723612</a>, Human Antithrombin III Rabbit mAb [PSH14-23]<br />Detector: <a href="/products/HA723613" style="font-weight: bold;text-decoration: underline;">HA723613</a>, Human Antithrombin III Rabbit mAb [PSH14-24]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA723612" style="font-weight: bold;text-decoration: underline;">HA723612</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Antithrombin III protein (<a href="/products/HA210985" style="font-weight: bold;text-decoration: underline;">HA210985</a>) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA723613" style="font-weight: bold;text-decoration: underline;">HA723613</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA723614B_1.png?v=20260604174806)
Sandwich ELISA analysis of Human Antithrombin III matched pair antibodies
Capture: HA723612, Human Antithrombin III Rabbit mAb [PSH14-23]
Detector: HA723613, Human Antithrombin III Rabbit mAb [PSH14-24]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723612) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human Antithrombin III protein (HA210985) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA723613, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
![Interpolated concentrations of native Antithrombin III in HepG2 culture supernatant and Human urine.<br /><br />Capture: <a href="/products/HA723612" style="font-weight: bold;text-decoration: underline;">HA723612</a>, Human Antithrombin III Rabbit mAb [PSH14-23]<br />Detector: <a href="/products/HA723613" style="font-weight: bold;text-decoration: underline;">HA723613</a>, Human Antithrombin III Rabbit mAb [PSH14-24]<br /><br />Interpolated concentration of native Antithrombin III was measured in duplicate at different sample concentrations and interpolated from the Antithrombin III standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 37019 pg/ml in HepG2 cell culture supernatant, 1364 pg/ml in Human urine.](https://storage.huabio.cn/huabio/productImg/HA723614B_2.png?v=20260604174806)
Interpolated concentrations of native Antithrombin III in HepG2 culture supernatant and Human urine.
Capture: HA723612, Human Antithrombin III Rabbit mAb [PSH14-23]
Detector: HA723613, Human Antithrombin III Rabbit mAb [PSH14-24]
Interpolated concentration of native Antithrombin III was measured in duplicate at different sample concentrations and interpolated from the Antithrombin III standard curves. The interpolated dilution factor corrected values were plotted (mean +/- SD, n=2). The mean Antithrombin III concentration was determined to be 37019 pg/ml in HepG2 cell culture supernatant, 1364 pg/ml in Human urine. -
![Interpolated concentrations of spiked Antithrombin III in cell culture media samples.<br /><br />Capture: <a href="/products/HA723612" style="font-weight: bold;text-decoration: underline;">HA723612</a>, Human Antithrombin III Rabbit mAb [PSH14-23]<br />Detector: <a href="/products/HA723613" style="font-weight: bold;text-decoration: underline;">HA723613</a>, Human Antithrombin III Rabbit mAb [PSH14-24]<br /><br />The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).](https://storage.huabio.cn/huabio/productImg/HA723614B_3.png?v=20260604174806)
Interpolated concentrations of spiked Antithrombin III in cell culture media samples.
Capture: HA723612, Human Antithrombin III Rabbit mAb [PSH14-23]
Detector: HA723613, Human Antithrombin III Rabbit mAb [PSH14-24]
The concentrations of Antithrombin III were measured in duplicates, interpolated from the Antithrombin III standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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