CD11b Mouse Monoclonal Antibody [4G2]
Catalog# RT1107
CD11b Mouse Monoclonal Antibody [4G2]
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WB
-
IP
-
IF
-
FC
-
Human
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Mouse
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unconjugated
Overview
Product Name
CD11b Mouse Monoclonal Antibody [4G2]
Antibody Type
Mouse Monoclonal Antibody
Immunogen
peptide
Species Reactivity
Human, Mouse
Validated Applications
WB, IP, IF, FC
Molecular Weight
170kDa
Positive Control
PBL, RAW 264.7.
Conjugation
unconjugated
Clone Number
4G2
Product Features
Form
Liquid
Concentration
Storage Instructions
Store at +4℃
Storage Buffer
1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
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IP
-
1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
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IF
-
1:50-500
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FC
-
1 μg per 1 x 106 cells
Target
Function
Integrin ?M, also designated complement component receptor-3 ?, CD11b (p170), macrophage antigen a polypeptide, cell surface glycoprotein Mac-1 a subunit, MAC1A, MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin ?M combines with the Integrin ∫2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin ?M/∫2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Background References
1. Nueda, A., et al. 1995. Hematopoietic cell-type-dependent regulation of leukocyte integrin functional activity: CD11b and CD11c expression inhibits LFA-1-dependent aggregation of differentiatied U937 cells. Cell. Immunol. 164: 163-169.
2. Li, R., et al. 1995. A peptide derived from the intercellular adhesion molecule-2 regulates the avidity of the leukocyte Integrins CD11b/CD18 and CD11c/CD18. J. Cell Biol. 129: 1143-115
Subcellular Location
Membrane
UNIPROT
Synonyms
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
ExpandImages
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Western blot analysis of Integrin αM expression in human PBL whole cell lysate immunoprecipitated with Integrin αM (2LPM19c) and detected with Integrin αM (H-61).
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Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing membrane staining.
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FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG1.
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FCM analysis of human peripheral blood leukocytes. Black line histogram represents the isotype control, normal mouse IgG1.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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