CD11b Recombinant Rabbit Monoclonal Antibody
Catalog# IRS014
CD11b Recombinant Rabbit Monoclonal Antibody
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mIHC
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Human
-
unconjugated
Overview
Product Name
CD11b Recombinant Rabbit Monoclonal Antibody
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human CD11b 1103-1152 / 1152.
Species Reactivity
Human
Validated Applications
mIHC
Molecular Weight
Predicted band size: 127 kDa
Positive Control
Human gastric cancer tissue, human liver tissue.
Conjugation
unconjugated
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
mIHC
-
1:100
Target
Function
Integrin αM, also designated complement component receptor-3 α, CD11b (p170), macrophage antigen a polypeptide, cell surface glycoprotein Mac-1 a subunit, MAC1A, MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin αM combines with the Integrin β2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin αM/β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.
Background References
1. Yu F et al. Repetitive Model of Mild Traumatic Brain Injury Produces Cortical Abnormalities Detectable by Magnetic Resonance Diffusion Imaging, Histopathology, and Behavior. J Neurotrauma 34:1364-1381 (2017).
2. Surolia R et al. 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs. JCI Insight 2:e91377 (2017).
Sequence Similarity
Belongs to the integrin alpha chain family.
Tissue Specificity
Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).
Subcellular Location
Cell membrane, Membrane raft.
UNIPROT
Synonyms
antigen CD11b (p170) antibody
Antigen CD11b p170 antibody
CD11 antigen like family member B antibody
CD11 antigen-like family member B antibody
CD11b antibody
CD11b/CD18 antibody
CD49d antibody
Cell surface glycoprotein MAC-1 subunit alpha antibody
Complement component 3 receptor 3 subunit antibody
Complement Component Receptor 3 Alpha antibody
ExpandImages
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mIHC analysis of human gastric cancer tissue (Formalin/PFA-fixed paraffin-embedded sections) with CD11b (IRS014), CD31 (IRS023), aSMA (IRS012), panCK (IRS010) and CD3 (IRS022) antibody at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900802). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
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mIHC analysis of human liver tissue (Formalin/PFA-fixed paraffin-embedded sections) with Rabbit anti-CD11b antibody (IRS014) at 1/100 dilution. The immunostaining was performed with the IRISKitCmTSA Kit (900808). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins at 95℃. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Olympus VS200 Slide Scanner.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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