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CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96] - BSA and Azide free

Usd: 649

Specification

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Catalog# HA750921

CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96] - BSA and Azide free

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

With BSA and Azide
Conjugation

  • unconjugated

Overview

Product Name

CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96] - BSA and Azide free

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD11b aa 1-100.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 127 kDa

Positive Control

TF-1 cell lysate, THP-1 cell lysate, U-937 cell lysate, RAW264.7 cell lysate, J774A.1 cell lysate, Mouse spleen tissue lysate, Rat spleen tissue lysate, human cervical cancer tissue, human spleen tissue, human tonsil tissue, mouse spleen tissue, rat spleen tissue.

Conjugation

unconjugated

Clone Number

PSH03-96

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:5,000-1:20,000

  • IF-Tissue

  • 1:1,000

Target

Function

Integrin αM, also designated complement component receptor-3 α, CD11b (p170), macrophage antigen a polypeptide, cell surface glycoprotein Mac-1 a subunit, MAC1A, MO1A and ITGAM) is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin αM combines with the Integrin β2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin αM/β2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.

Background References

1. Yu F et al. Repetitive Model of Mild Traumatic Brain Injury Produces Cortical Abnormalities Detectable by Magnetic Resonance Diffusion Imaging, Histopathology, and Behavior. J Neurotrauma 34:1364-1381 (2017).

2. Surolia R et al. 3D pulmospheres serve as a personalized and predictive multicellular model for assessment of antifibrotic drugs. JCI Insight 2:e91377 (2017).

Subcellular Location

Cell membrane, Membrane raft.

UNIPROT

SwissProt: P11215 Human

SwissProt: P05555 Mouse

Entrez Gene: 25021 Rat

Synonyms

antigen CD11b (p170) antibody

Antigen CD11b p170 antibody

CD11 antigen like family member B antibody

CD11 antigen-like family member B antibody

CD11b antibody

CD11b/CD18 antibody

CD49d antibody

Cell surface glycoprotein MAC-1 subunit alpha antibody

Complement component 3 receptor 3 subunit antibody

Complement Component Receptor 3 Alpha antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/2,000 dilution.<br /><br />Lane 1: TF-1 cell lysate (10 µg/Lane)<br />Lane 2: THP-1 cell lysate (15 µg/Lane)<br />Lane 3: U-937 cell lysate (30 µg/Lane)<br />Lane 4: Jurkat cell lysate (negative) (10 µg/Lane)<br />Lane 5: RAW264.7 cell lysate (5 µg/Lane)<br />Lane 6: J774A.1 cell lysate (5 µg/Lane)<br /><br />Predicted band size: 127 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 59 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA750921) at 1/2,000 dilution.

    Lane 1: TF-1 cell lysate (10 µg/Lane)
    Lane 2: THP-1 cell lysate (15 µg/Lane)
    Lane 3: U-937 cell lysate (30 µg/Lane)
    Lane 4: Jurkat cell lysate (negative) (10 µg/Lane)
    Lane 5: RAW264.7 cell lysate (5 µg/Lane)
    Lane 6: J774A.1 cell lysate (5 µg/Lane)

    Predicted band size: 127 kDa
    Observed band size: 170 kDa

    Exposure time: 59 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750921) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse spleen tissue lysate<br />Lane 2: Mouse heart tissue lysate (negative)<br />Lane 3: Rat spleen tissue lysate<br />Lane 4: Rat heart tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 127 kDa<br />Observed band size: 170 kDa<br /><br />Exposure time: 12 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA750921) at 1/2,000 dilution.

    Lane 1: Mouse spleen tissue lysate
    Lane 2: Mouse heart tissue lysate (negative)
    Lane 3: Rat spleen tissue lysate
    Lane 4: Rat heart tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 127 kDa
    Observed band size: 170 kDa

    Exposure time: 12 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750921) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue with Rabbit anti-CD11b antibody (HA750921) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue with Rabbit anti-CD11b antibody (HA750921) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD11b antibody (HA750921) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rabbit anti-CD11b antibody (HA750921) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue with Rabbit anti-CD11b antibody (HA750921) at 1/20,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA750921) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD11b with Rabbit anti-CD11b antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (<a href="/products/HA750921" style="font-weight: bold;text-decoration: underline;">HA750921</a>, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

    Immunofluorescence analysis of paraffin-embedded mouse spleen tissue labeling CD11b with Rabbit anti-CD11b antibody (HA750921) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA750921, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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