CD11b Recombinant Rabbit Monoclonal Antibody [PSH03-96] (HA722075)
Catalog# HA722075
Rabbit Monoclonal to CD11b
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CD11b on different lysates with Rabbit anti-CD11b antibody (HA722075) at 1/2,000 dilution.
Lane 1: TF-1 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (15 µg/Lane)
Lane 3: U-937 cell lysate (30 µg/Lane)
Lane 4: Jurkat cell lysate (negative) (10 µg/Lane)
Lane 5: RAW264.7 cell lysate (5 µg/Lane)
Lane 6: J774A.1 cell lysate (5 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: TF-1 cell lysate (10 µg/Lane)
Lane 2: THP-1 cell lysate (15 µg/Lane)
Lane 3: U-937 cell lysate (30 µg/Lane)
Lane 4: Jurkat cell lysate (negative) (10 µg/Lane)
Lane 5: RAW264.7 cell lysate (5 µg/Lane)
Lane 6: J774A.1 cell lysate (5 µg/Lane)
Predicted band size: 127 kDa
Observed band size: 170 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722075) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.